# US 5,348,856 A · DNA encoding TRKC protein > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,348,856 A - Document ID: US5348856A - Status: Granted - Kind: A - Application number: 07/912,952 - Filed: 1992-07-07 - Granted: 1994-09-20 - Earliest claimed: 1991-07-08 - Assignees: E. R. Squibb & Sons, Inc. - Inventors: Mariano Barbacid; Fabienne Lamballe - Web page: https://patentgrep.com/patent/US5348856A ## CPC - C07K 14/82: Translation products from oncogenes - C07K 14/71: for growth factors; for growth regulators - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract Nucleic acid sequences, particularly DNA sequences, coding for all or part of a trkC protein, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods utilizing these materials. The invention also concerns polypeptide molecules comprising all or part of a trkC protein and methods for producing these polypeptide molecules. ## Claims ### Claim 1 An isolated nucleic acid molecule coding for a trkC protein wherein said nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleic acid sequence coding for the amino acid sequence of FIGS 1B and 1C (SEQ. ID. NO.:2) and (b) a nucleic acid capable of hybridizing to the complement of a nucleic acid according to (a) above under stringent conditions. ### Claim 2 (depends on 1) The nucleic acid molecule according to claim 1 which is a DNA molecule and wherein the nucleic acid sequence is a DNA sequence. ### Claim 3 (depends on 2) The DNA molecule according to claim 2 wherein the encoded trkC protein is TrkC K1, TrkC K2, TrkC K3, TrkC NC1 or TrkC NC2. ### Claim 4 (depends on 2) The DNA molecule according to claim 2 wherein the DNA sequence has the nucleotide sequence as shown in FIG. 1B (SEQ. ID NO: 1). ### Claim 5 (depends on 2) The DNA molecule according to claim 2 wherein the DNA sequence has the nucleotide sequence of the 2.4 kb EcoRI insert of pFL16 deposited with the American Type Culture Collection. ### Claim 6 (depends on 4, 5) A DNA molecule having a DNA sequence which is complementary to the DNA sequence according to claims 4 or 5. ### Claim 7 An expression vector comprising a DNA coding for a trkC protein wherein said DNA is selected from the group consisting of: (a) a DNA having a sequence coding for the amine acid sequence of FIGS. 1B and 1C (SEQ. ID. NO.: 2) and (b) a DNA capable of hybridizing to the complement of a DNA according to (a) above under stringent conditions. ### Claim 8 (depends on 7) The expression vector according to claim 7 comprising one or more control DNA sequences capable of directing the replication and/or the expression of and operatively linked to the DNA coding for a trkC protein. ### Claim 9 (depends on 7) The expression vector according to claim 7 wherein the DNA coding for a trkC protein has the nucleotide sequence substantially as shown in FIG. 1B (SEQ. ID NO: 1). ### Claim 10 (depends on 7) The expression vector according to claim 7 wherein the DNA sequence coding for the amino acid sequence of FIGS. 1B and 1C (SEQ. ID. NO.: 2) has the nucleotide sequence shown in FIGS. 1B and 1C (SEQ. ID NO: 1). ### Claim 11 (depends on 7) The expression vector according to claim 7 wherein the DNA coding for a trkC protein has the nucleotide sequence of the 2.4 kb EcoRI insert of pFL16 deposited with the American Type Culture Collection. ### Claim 12 (depends on 7) The expression vector according to claim 7 designated pFL19. ### Claim 13 (depends on 7) The expression vector according to claim 7 designated pFL16. ### Claim 14 (depends on 7) The expression vector according to claim 7 wherein the encoded trkC protein is TrkC K1, TrkC K2, TrkC K3, TrkC NC1 or TrkC NC2. ### Claim 15 (depends on 8, 9, 10, 11, 12, 13, 14) A prokaryotic or eukaryotic host cell containing the expression vector according to claim 8, 9, 10, 11, 12, 13 or 14. ### Claim 16 (depends on 7) A prokaryotic or eukaryotic host cell containing the expression vector according to claim 7. ### Claim 17 (depends on 16) A method for producing a polypeptide molecule which comprises a trkC protein comprising culturing a host cell according to claim 16 under conditions permitting expression of the polypeptide molecule. ### Claim 18 A method for detecting a nucleic acid encoding all or part of a trkC protein or a related nucleic acid comprising contacting the nucleic acid with a detectable marker having a sequence of at least 15 sequential nucleotides complementary to the nucleic acid sequence of FIGS. 1B and 1C (SEQ. ID. NO.: 1 ) under conditions which permit binding of said nucleic acid to said marker and detecting the marker so bound, the presence of bound marker indicating the presence of the nucleic acid.