# US 5,348,675 A · Heteropolysaccharide BM07

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- Number: US 5,348,675 A
- Document ID: US5348675A
- Status: Granted
- Kind: A
- Application number: 08/005,572
- Filed: 1993-01-19
- Granted: 1994-09-20
- Earliest claimed: 1988-07-13
- Assignees: Rhone-Poulenc Chimie
- Inventors: Gabriel Ullmann; Alain Jarry
- Web page: https://patentgrep.com/patent/US5348675A

## CPC

- C23G 1/025: {acidic pickling pastes}
- C08B 37/006: {Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof}
- C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- Y10S 435/822: using bacteria or actinomycetales
- C23G: CLEANING OR DE-GREASING OF METALLIC MATERIAL BY CHEMICAL METHODS OTHER THAN ELECTROLYSIS
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

The novel heteropolysaccharide BM07, useful, e.g., as a thickening/stabilizing/suspending agent for a wide variety of industrial applications, is produced by fermenting a nutrient medium containing at least one source of assimilable carbon with a strain of Agrobacterium tumefaciens I-736, or recombinant or mutant thereof.

## Claims

### Claim 1

A process for the preparation of heteropolysaccharide BM07, comprising fermenting a nutrient medium containing at least one source of assimilable carbon by a strain of Agrobacterium tumefaciens I-736, or one of the mutants thereof and recovering said heteropolysaccharide BM07 from said medium.

### Claim 2 (depends on 1)

The process as defined by claim 1, said at least one source of assimilable carbon comprising glucose, saccharose or a starch hydrolysate.

### Claim 3 (depends on 1)

The process as defined by claim 1, wherein the concentration of the at least one source of assimilable carbon in the fermentation medium ranges from 1 to 100 g/l.

### Claim 4 (depends on 1)

The process as defined by claim 1, said fermentation medium comprising at least one source of organic nitrogen.

### Claim 5 (depends on 4)

The process as defined by claim 4, said at least one source of organic nitrogen comprising casein, a caseinate, wheat, corn or soybean flour, a yeast extract, a dry distiller's soluble, potato protein, corn steep liquor or solubles of corn steep liquor.

### Claim 6 (depends on 5)

The process as defined by claim 5, said at least one source of organic nitrogen comprising corn steep liquor or soluble of corn steep liquor.

### Claim 7 (depends on 4)

The process as defined by claim 4, wherein the concentration of the at least one source of organic nitrogen in the fermentation medium ranges from 3 to 80 g/l.

### Claim 8 (depends on 1)

The process as defined by claim 1, said fermentation medium comprising at least one inorganic salt.

### Claim 9 (depends on 8)

The process as defined by claim 8, said at least one inorganic salt comprising a sulfate or a carbonate.

### Claim 10 (depends on 8)

The process as defined by claim 8, wherein the concentration of said at least one inorganic salt ranges from 0.01 to 5 g/l.

### Claim 11 (depends on 1)

The process as defined by claim 1, carried out at a pressure ranging from 1 to 4 bar and at a temperature ranging from 25.degree. C. to 35.degree. C., under submerged aerobic conditions.

### Claim 12 (depends on 1)

The process as defined by claim 1, wherein the pH of the fermentation medium ranges from 5 and 9.

### Claim 13 (depends on 1)

The process as defined by claim 1, wherein the fermentation yield ranges from 40% to 75% by weight of the heteropolysaccharide BM07 relative to the at least one carbon source.

### Claim 14 (depends on 1)

The process as defined by claim 1, comprising separating the heteropolysaccharide BM07 from the fermentation wort by (a) heating the wort to 80.degree. to 120.degree. C. for 10 to 60 min, (b) precipitating the heteropolysaccharide BM07 with an organic liquid that is miscible with water, and (c) separating the heteropolysaccharide BM07 from the organic liquid by filtration, centrifugation or draining.

### Claim 15 (depends on 14)

The process as defined by claim 14, said organic liquid comprising ethanol, propanol, isopropanol, butanol or tert-butanol.

### Claim 16 (depends on 14)

The process as defined by claim 14, further comprising dehydrating, drying, grinding and classifying said separated heteropolysaccharide BM07.

### Claim 17

A thickening agent comprising the heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon, by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof.

### Claim 18

A suspending agent comprising the heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon, by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof.

### Claim 19

A stabilizing agent comprising the heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon, by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof.

### Claim 20

A composition of matter comprising an aqueous solution of an organic acid, strong base and/or salt of an organic or inorganic acid, said aqueous solution containing a thickening amount of the heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon, by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof.

### Claim 21

In a process for the thickening of an aqueous medium, the improvement which comprises utilizing as the thickening agent therefor, an effective amount of the heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon, by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof.

### Claim 22

In a process for the cleaning or descaling of a substrate surface, the improvement which comprises including in the cleaning/descaling composition therefor, the heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon, by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof.

### Claim 23

The heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon, by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof, having an intrinsic viscosity ranging from 140 to 250 dl/g.

### Claim 24 (depends on 23)

The heteropolysaccharide BM07 as defined by claim 23, having an intrinsic viscosity ranging from 150 to 240 dl/g.

### Claim 25

A solution comprising (i) 0.1% by weight of a heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon by a strain of Agrobacterium tumefaciens I-756, or one of the recombinants or mutants thereof, and (ii) distilled water, said solution having a viscosity at 25.degree. C. after 24 hours ranging from 400 to 700 mPa.s.

### Claim 26

A solution comprising (i) 0.2% by weight of a heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof, and (ii) a 20% aqueous solution of NaCl, said solution having a viscosity after 24 hours ranging from 1,700 to 2,100 mPa.s.

### Claim 27

An aqueous solution comprising 0.2% by weight of a heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon, by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof at pH, said solution having a viscosity at pH 7 and 25.degree. C. ranging from 1,400 to 2,000 mPa.s.

### Claim 28

An aqueous solution comprising 0.2% by weight of a heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon, by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof, said solution having a viscosity at pH 1.7 and 25.degree. C. after 24 hours ranging from 1,400 to 2,000 mPa.s.

### Claim 29

An aqueous solution comprising 0.2% by weight of a heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon, by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof, said solution having a viscosity at pH 11.8 and 25.degree. C. after 24 hours ranging from 1,400 to 2,000 mPa.s.

### Claim 30

A solution comprising (i) 0.2% by weight of a heteropolysaccharide BM07 produced by fermentation of a medium comprising at least one source of assimilable carbon by a strain of Agrobacterium tumefaciens I-736, or one of the recombinants or mutants thereof and (ii) distilled water, said solution being subjected to a temperature of 80.degree. C. for 24 hours and having a viscosity ranging from 1,000 to 2,000 mPa.s.

### Claim 31 (depends on 3)

The process as defined by claim 3, said concentration ranging from 15 to 60 g/l.