# US 5,348,649 A · Apparatus for measuring glycohemoglobin

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- Number: US 5,348,649 A
- Document ID: US5348649A
- Status: Granted
- Kind: A
- Application number: 08/059,931
- Filed: 1993-05-11
- Granted: 1994-09-20
- Earliest claimed: 1990-07-20
- Assignees: Hitachi, Ltd.
- Inventors: Masako Mizuno; Kenji Tochigi; Yutaka Misawa
- Web page: https://patentgrep.com/patent/US5348649A

## CPC

- B01D 15/08: Selective adsorption, e.g. chromatography
- B01D 15/22: relating to the construction of the column
- B01J 39/07: in the weakly acidic form
- B01J 39/26: Cation exchangers for chromatographic processes
- B01J 47/018: Granulation; Incorporation of ion-exchangers in a matrix; Mixing with inert materials
- G01N 30/88: Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N 33/723: {Glycosylated haemoglobin}
- G01N 2030/8813: {biological materials}
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

In separation column 23 is packed a filler consisting of an inorganic or organic porous substance having carboxyalkyl group and dihydroxyboronyl group. Thus, the separation column 23 can concurrently perform the procedure of separating glycohemoglobin, hemoglobin and hemoglobin derivatives in blood and the procedure of decomposing labile-type hemoglobin in blood into glucose and hemoglobin to remove the labile-type hemoglobin, when a diluted blood sample on sample table 19 along with eluents 8 to 10 is transferred to the separation column 23. Then, stable-type hemoglobin in the eluate from the separation column 23 is measured by UV-VIS detector 25.

## Claims

### Claim 1

An apparatus for measuring glycohemoglobin to measure stable-type hemoglobin by high performance liquid chromatography, comprising a separation column for separating glycohemoglobin in blood, a means for injecting a diluted blood sample into the separation column, a means for passing eluents into the separation column and a means for detecting stable-type hemoglobin in an eluate from the separation column, wherein the separation column is provided with filler means having carboxyalkyl groups and dihydroxyboronyl groups for simultaneously carrying out the procedure of separating glycohemoglobin, hemoglobin and hemoglobin derivatives and the procedure of decomposing labile-type hemoglobin in the blood into glucose and hemoglobin so as to remove the labile-type hemoglobin.

### Claim 2

An apparatus for measuring glycohemoglobin to measure stable-type hemoglobin by high-performance liquid chromatography, comprising a separation column for separating glycohemoglobin in blood, a means for injecting a diluted blood sample into the separation column, a means for passing eluents into the separation column and a means for detecting stable-type hemoglobin in an eluate from the separation column, wherein the separation column is packed with a filler consisting of an inorganic or organic porous substance having carboxyalkyl group and dihydroxyboronyl group.

### Claim 3

An apparatus for measuring glycohemoglobin to measure stable-type hemoglobin by high-performance liquid chromatography, comprising a separation column for separating glycohemoglobin in blood, a means for injecting a diluted blood sample into the separation column, a means for passing eluents into the separation column and a means for detecting stable-type hemoglobin in an eluate from the separation column, wherein the separation column is packed with a filler produced by mixing a filler consisting of an inorganic or organic porous substance having carboxyalkyl group and a filler consisting of an inorganic or organic porous substance having dihydroxyboronyl group.

### Claim 4

An apparatus for measuring glycohemoglobin to measure stable-type hemoglobin in blood by high-performance liquid chromatography, comprising a separation column for separating glycohemoglobin in blood, a means for injecting a diluted blood sample into the separation column, a means for passing eluents into the separation column and a means for detecting stable-type hemoglobin in an eluate from the separation column, wherein a filler consisting of an inorganic or organic porous substance having carboxyalkyl group and a filler consisting of an inorganic or organic porous substance having dihydroxyboronyl group are separated in two layers and packed individually in the separation column.

### Claim 5

An apparatus for measuring glycohemoglobin to measure stable-type hemoglobin by high-performance liquid chromatography, comprising a separation column for separating glycohemoglobin in blood, a means for injecting a diluted blood sample into the separation column, a means for passing eluents into the separation column and a means for detecting stable-type hemoglobin in an eluate from the separation column, wherein the separation column is divided in two parts in the flow direction of eluents, one part being packed with a filler consisting of an inorganic or organic porous substance having carboxyalkyl group and the other part being packed with a filler consisting of an inorganic or organic substance having dihydroxyboronyl group.

### Claim 6

An apparatus for measuring glycohemoglobin to measure stable-type hemoglobin by high-performance liquid chromatography, comprising a separation column for separating glycohemoglobin in blood, a means for injecting a diluted blood sample into the separation column, a means for passing eluents into the separation column and a means for detecting stable-type hemoglobin in an eluate from the separation column, wherein the separation column is divided with filler means for simultaneously carrying out the procedure of separating glycohemoglobin, hemoglobin and hemoglobin derivatives and the procedure of decomposing labile-type hemoglobin in the blood into glucose and hemoglobin so as to remove the labile-type hemoglobin.