# US 5,348,606 A · Method of making a multiple pipette sampler system > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,348,606 A - Document ID: US5348606A - Status: Granted - Kind: A - Application number: 07/950,967 - Filed: 1992-09-23 - Granted: 1994-09-20 - Earliest claimed: 1986-10-24 - Inventors: Richard W. Hanaway; James Hrudicka - Web page: https://patentgrep.com/patent/US5348606A ## CPC - B01L 3/021: {Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids} - G01N 35/1065: {Multiple transfer devices} - G01N 2035/00237: {Handling microquantities of analyte, e.g. microvalves, capillary networks} - Y10T 156/1002: with permanent bending or reshaping or surface deformation of self sustaining lamina - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract A method of making a pipette sampler system for removing and transferring a plurality of liquid samples includes the step of thermoforming a plurality of identical pipettes in a first thin PVC plastic sheet utilizing a first precision die. A concave housing including a plurality of stiffening depressions and a skirt portion possessing a peripheral sealing edge is thermoformed from a second thin PVC plastic sheet utilizing a second die. The housing and pipette sheet are joined by dielectric heat sealing forming a manifold communicating with each pipette, with the peripheral sealing edge and each of the stiffening depressions heat welded to the pipette sheet, forming an inflexible rigid monocoque structure. A filling tray is thermoformed from a third thin PVC plastic sheet utilizing a third die. A sealing washer is sealed to a top surface of the housing, surrounding an aperture formed through a top surface of the housing and communicating with the manifold. The sealing washer provides a sealing surface for attachment of an actuator which selectively provides a vacuum in the manifold to draw liquid into the pipettes from the filling tray. ## Claims ### Claim 1 A method of making a multiple pipette sampler device comprising the steps of: forming a plurality of pipettes in a rectangular array in a thin plastic pipette sheet; forming a concavely-shaped housing including an interiorly formed step from a thin plastic sheet; forming a plurality of stiffening depressions in a top surface of said housing; joining said housing to said pipette sheet by substantially permanently sealing a peripheral edge of said pipette sheet to said step, creating a manifold in fluid communication with each of said pipettes and also creating a rigid monocoque structure for maintaining said pipette sheet in a substantially planar configuration; disposing a bottom portion of at least one of said stiffening depressions in abutment with said pipette sheet; and substantially permanently securing said bottom portion of said at least one of said stiffening depressions to said pipette sheet. ### Claim 2 (depends on 1) The method of claim 1 wherein said housing and said pipette sheet are thermoformed from a plastic sheet material possessing a thickness in the range of 0.010 to 0.030 inches. ### Claim 3 (depends on 1) The method of claim 1 wherein each of said pipettes is thermoformed in a half hour-glass configuration having a larger diameter upper portion connected by a substantially circular rim with said pipette sheet, each of said pipettes tapering to a small diameter tip portion forming a capillary tube. ### Claim 4 (depends on 1) The method of claim 1 further comprising the step of forming an attachment means in said manifold whereby a pressure varying means may be joined physically and pneumatically to said manifold, thereby allowing said pipette sheet, said housing, and said pressure varying means to be easily transported together during usage of said multiple pipette sampler system. ### Claim 5 (depends on 1) The method of claim 1 wherein said step of sealing a peripheral edge of said pipette sheet to said step includes dielectric heat sealing. ### Claim 6 (depends on 1) The method of claim 1 wherein said step of securing said bottom portion of said at least one of said stiffening depressions to said pipette sheet includes the step of heat welding said bottom portion to said pipette sheet. ### Claim 7 (depends on 1) The method of claim 1 further comprising the steps of forming a plurality concave stiffening depressions in a uniform rectangular array in a top surface of said housing; disposing a bottom portion of each of said stiffening depressions in abutment with said pipette sheet; and substantially permanently securing said bottom portions of each of said stiffening depressions to said pipette sheet, forming beam structures for rigidly maintaining positions of said pipettes during pressure variations in said manifold. ### Claim 8 (depends on 7) The method of claim 7 wherein said step of securing said bottom portions of said stiffening depressions to said pipette sheet includes the step of heat welding said bottom portions to said pipette sheet. ### Claim 9 (depends on 1) The method of claim 1 further comprising the step of forming an outwardly flaring peripheral skirt on a bottom portion of said housing. ### Claim 10 (depends on 9) The method of claim 9 further comprising the step of forming a filling tray from a plastic sheet, said filling tray having upwardly extending side wall portions dimensioned for engagement with said housing skirt; and forming a plurality of concave liquid holding troughs in said tray, said troughs disposed to receive said pipettes when said housing is placed over said tray.