# US 5,347,951 A · Process for producing pearls in abalone and other shell-bearing molluska and nucleus used therewith > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,347,951 A - Document ID: US5347951A - Status: Granted - Kind: A - Application number: 08/082,496 - Filed: 1993-06-28 - Granted: 1994-09-20 - Earliest claimed: 1990-11-15 - Assignees: Pacific Pearl Culture Ltd. - Inventors: Peter V. Fankboner - Web page: https://patentgrep.com/patent/US5347951A ## CPC - A01K 61/54: of bivalves, e.g. oysters or mussels - Y02A 40/81: Aquaculture, e.g. of fish - A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract The process includes the step of forming an opening in the shell of a host mollusk, such as an abalone, at a location covering a soft tissue thereof, such as the mantle-covered gonadal tissue. A pearl nucleus is then inserted into the opening. The pearl nucleus comprises a nucleating portion, typically semispherical in configuration, and a nut portion. The pearl nucleus is either threaded or configured to provide a tight frictional fit with the peripheral edge of the opening. The pearl nucleus is inserted into the opening and then turned by means of the nut portion to provide a very tight fit in the opening. In another embodiment, a pearl nucleus is drawn into the abalone in the vicinity of the head region, or through an opening formed in the abalone shell, by means of a probe which is introduced into the abalone through another smaller opening. The pearl nucleus is pulled into the abalone until it is seated adjacent the smaller opening, where it is held by tissue pressure and glue. The larger opening drilled in the abalone shell may be closed with an elongate pearl nucleus having a size and shape sufficient to pass through the opening in a first orientation and capable of substantially occluding the opening in a second orientation. In a further alternative embodiment, a pearl nucleus and piece of graft tissue are secured to one end of a curved, elongate probe and threaded through a soft tissue of the abalone to an implanted position. The same procedure may be employed for implanting graft tissue absent a pearl nucleus to form non-nucleated pearls. In a further alternative embodiment of the process, a pearl nucleus may be formed in a soft tissue of an abalone, such as the aductor muscle, by using a hypodermic syringe to inject a fast-setting resin into a cavity formed in the muscle tissue. ## Claims ### Claim 1 A process for nucleating pearls in shell-bearing molluska including abalone, comprising the steps of: (a) forming an opening in the shell of a host mollusk in a region covering a soft tissue thereof, said opening having a peripheral edge extending between an exterior surface and an interior surface of said shell; (b) providing a pearl nucleus having a first portion around which nacre forms and a second portion securely connected to said first portion and having a region larger than the size of said opening; and (c) manually inserting said nucleus through said opening to an implanted position wherein said nucleus first portion is compressed against said soft tissue and said nucleus second portion physically engages said peripheral edge of said opening with a sufficient degree of force to prevent expulsion of said nucleus by said host mollusk or other molluska. ### Claim 2 (depends on 1) A process as defined in claim 1, wherein said pearl nucleus is inserted through said opening by turning said nucleus about a longitudinal axis thereof. ### Claim 3 (depends on 2) A process as defined in claim 2, wherein an outer end of said nucleus second portion furthest from said nucleus first portion has an outline which is mateable with a tool for manually turning said nucleus. ### Claim 4 (depends on 2) A process as defined in claim 2, wherein said nucleus second portion further comprises an externally threaded surface for threadingly engaging said peripheral edge of said opening. ### Claim 5 (depends on 1) A process as defined in claim 1, wherein an outer end of said nucleus second portion furthest from said nucleus first portion extends outwardly of said shell in said implanted position. ### Claim 6 (depends on 5) A process as defined in claim 5, wherein said outer end of said nucleus second portion comprises a nut-like portion. ### Claim 7 (depends on 6) A process as defined in claim 6, wherein said nut-like portion is larger than the size of said opening in said shell. ### Claim 8 (depends on 6) A process as defined in claim 6, wherein said nut-like portion has an outline which is mateable with a tool for manually turning said nucleus. ### Claim 9 (depends on 6) A process as defined in claim 6, wherein said nut-like portion is integral with the remainder of said pearl nucleus. ### Claim 10 (depends on 6) A process as defined in claim 6, wherein said nut-like portion is separate from but secured to the remainder of said pearl nucleus. ### Claim 11 (depends on 1) A process as defined in claim 1, wherein an outer end of said nucleus second portion furthest from said nucleus first portion extends substantially flush with said shell exterior surface in said implanted position. ### Claim 12 (depends on 1) A process as defined in claim 1, wherein said first and second nucleus portions are rigidly coupled together. ### Claim 13 (depends on 1) A process as defined in claim 1, wherein said pearl nucleus second portion further comprises outwardly sloping side surfaces for frictionally engaging said peripheral edge of said opening. ### Claim 14 (depends on 1) A process as defined in claim 1, wherein said opening is substantially round and wherein said opening is formed by passing a drill through said shell in a direction substantially perpendicular to said shell outer surface. ### Claim 15 (depends on 14) A process as defined in claim 14, further including the step of setting said drill to a preselected depth. ### Claim 16 (depends on 1) A process as defined in claim 1, wherein said soft tissue is the mantle-covered gonadal tissue of said mollusk. ### Claim 17 A pearl nucleus for use in nucleating shell-bearing molluska including abalone, said nucleus comprising: (a) a first portion around which nacre forms upon insertion of said pearl nucleus into the interior of a host mollusk through an opening formed in the shell thereof, said opening having a peripheral edge extending between an outer an inner surface of said shell; and (b) a plug-like second portion securely connected to said first portion, wherein said second portion comprises securing means formed on an external surface thereof for physically engaging said peripheral edge of said opening with a sufficient degree of force to prevent expulsion of said nucleus by said host mollusk or other molluska. ### Claim 18 (depends on 17) A pearl nucleus as defined in claim 17, wherein an outer end of said nucleus second portion furthest from said nucleus first portion is mateable with a tool for turning said nucleus. ### Claim 19 (depends on 18) A pearl nucleus as defined in claim 18, wherein said securing means comprises an externally threaded surface on said second portion for threadingly engaging said peripheral edge of said opening. ### Claim 20 (depends on 17) A pearl nucleus as defined in claim 17, wherein said securing means comprises sloped side surfaces on said second portion for frictionally engaging said peripheral edge of said opening. ### Claim 21 (depends on 20) A pearl nucleus as defined in claim 20, wherein said side surfaces are roughened to facilitate said frictional engagement. ### Claim 22 (depends on 17) A pearl nucleus as defined in claim 17, wherein said securing means comprises resilient annular rings encircling said nucleus second portion for engaging said peripheral edge of said opening. ### Claim 23 (depends on 17) A pearl nucleus as defined in claim 17, wherein said first and second nucleus portions are rigidly coupled together. ### Claim 24 (depends on 17) A pearl nucleus as defined in claim 17, wherein an outer end of said nucleus second portion furthest from said nucleus first portion comprises a nut-like portion. ### Claim 25 (depends on 24) A pearl nucleus as defined in claim 24, wherein said nut-like portion is larger than the size of said opening in said shell. ### Claim 26 (depends on 25) A pearl nucleus as defined in claim 25, wherein said nut-like portion is integral with the remainder of said pearl nucleus. ### Claim 27 (depends on 26) A pearl nucleus as defined in claim 26, wherein said nut-like portion is separate from but secured to the remainder of said pearl nucleus. ### Claim 28 A process for nucleating pearls in shell-bearing molluska including abalone, comprising the steps of: (a) forming an opening in the shell of a host mollusk in a region covering a soft tissue thereof; (b) inserting a pearl nucleus through said opening, wherein said pearl nucleus includes a nucleating portion and a disk portion which is substantially larger in diameter than said nucleating portion; (c) gluing said disk portion to said shell; and (d) clamping said disk portion in place until the glue sets by means of a clamp which is configured to fit around a portion of the said shell and which comprises end portions configured to grip the edges of said shell. ### Claim 29 A process for nucleating pearls in shell-bearing molluska including abalone, comprising the steps of: (a) forming an opening in the shell of a host mollusk in a region covering a soft tissue thereof; (b) inserting an elongated probe through said opening and passing said probe through an interior cavity of said mollusk between said soft tissue and said shell until a tip of said probe is exposed from said mollusk; (c) securing a filament to said exposed tip of said probe, said filament having a pearl nucleus secured thereto, wherein said nucleus is larger than the size of said opening; (d) withdrawing said probe and at least part of said filament through said opening thereby pulling said nucleus through said cavity until said nucleus is positioned against said soft tissue adjacent opening; and (e) securing said pearl nucleus to a portion of said shell adjacent said opening. ### Claim 30 (depends on 29) A process as defined in claim 29, wherein the step of securing comprises gluing said pearl nucleus to said shell. ### Claim 31 (depends on 29) A process as defined in claim 29, wherein said tip of said probe has an aperture formed therein and said filament is a flexible thread which is threaded through said aperture and tied to said tip, and wherein said filament is severed after said pearl nucleus has been positioned against said opening. ### Claim 32 (depends on 29) A process as defined in claim 29, wherein a plurality of openings are formed in said shell, and a plurality of nuclei are implanted within said mollusk, each of said nuclei being implanted adjacent one of said openings. ### Claim 33 (depends on 32) A process as defined in claim 32, wherein said plurality of openings are smaller than the size of said nuclei and wherein said process further includes the step of making an additional opening larger than the size of said nuclei through which said nuclei are consecutively pulled into said mollusk and then positioned against one of said plurality of openings. ### Claim 34 A process for nucleating spherical pearls in shell-bearing molluska including abalone, comprising the steps of: (a) forming an opening in the shell of a host mollusk in a region covering a soft tissue thereof; (b) inserting a pearl nucleus through said opening until at least part of said nucleus is securely compressed against said soft tissue; (c) allowing said pearl nucleus to remain in said mollusk for a sufficient period of time such said soft tissue in the vicinity of said pearl nucleus forms a cup-like cavity; (d) removing said pearl nucleus from said mollusk, thereby exposing said cavity formed in said soft tissue; (e) inserting a spherical pearl nucleus into said cavity; (f) suturing said soft tissue so as to close said tissue substantially around said spherical nucleus; and (g) closing said opening in said shell. ### Claim 35 (depends on 34) A process as defined in claim 34, wherein the step of suturing includes using a dissolving thread. ### Claim 36 (depends on 34) A process as defined in claim 34, further comprising the step of adding a small amount of fast-setting glue to said suture. ### Claim 37 (depends on 34) A process as defined in claim 34, wherein the step of closing includes gluing a disk over the opening formed in said shell. ### Claim 38 A process for nucleating pearls in shell-bearing molluska including abalone, comprising the steps of: (a) securing a pearl nucleus to one end of a thin filament; (b) securing the other end of said filament to a trailing end of an elongate probe; (c) threading said probe and said filament through a piece of graft tissue until said tissue is positioned immediately adjacent said nucleus; (d) forming a first opening in the shell of a host mollusk, said first opening having a size exceeding the size of said pearl nucleus; (e) forming a second opening in the shell of said mollusk spaced apart from said first opening; (f) inserting a leading end of said elongate probe through said first opening into a soft tissue of said mollusk and passing said probe through said soft tissue until said probe leading end emerges from said second opening; (g) withdrawing said probe and said filament from said mollusk through said second opening until said nucleus and said tissue graft become lodged within said soft tissue adjacent said second opening; and (h) severing said nucleus from said filament. ### Claim 39 (depends on 38) A process as defined in claim 38, wherein said elongate probe is arcuately curved. ### Claim 40 (depends on 39) A process as defined in claim 39, wherein said probe has a recess formed at said trailing end for receiving said other end of said filament. ### Claim 41 (depends on 38) A process as defined in claim 38, wherein said first and second openings are formed overlying the gonadal tissue of said mollusk and wherein said probe is passed through said gonadal tissue. ### Claim 42 (depends on 38) A process as defined in claim 38, wherein said second opening has a diameter smaller than said pearl nucleus. ### Claim 43 (depends on 38) A process as defined in claim 38, further comprising the steps of closing said first and second openings after said filament is severed from said pearl nucleus. ### Claim 44 A process for culturing non-nucleated free pearls in shell-bearing molluska including abalone, comprising the steps of: (a) loosely threading a thin filament through a piece of tissue graft; (b) securing the free ends of said filament to a trailing end of an elongate probe; (c) forming a first opening in the shell of a host mollusk in a region covering a soft tissue thereof; (d) forming a second opening in said shell of said mollusk spaced apart from said first opening but also overlying said soft tissue; (e) inserting a leading end of said probe through said first opening and passing said probe through said soft tissue until said probe leading end emerges from said second opening; (f) withdrawing said probe and part of said attached filament from said mollusk through said second opening; (g) severing said filament from said probe; and (h) removing said filament from said mollusk through said second opening by pulling one of the severed ends of said filament, thereby leaving said tissue graft embedded in said soft tissue. ### Claim 45 (depends on 44) A process as defined in claim 44 comprising a plurality of tissue grafts, each of said grafts having a thin filament loosely threaded therethrough, wherein the free ends of each of said filaments are secured within a cavity formed at said trailing end of said elongate probe. ### Claim 46 A process as defined in 45, wherein said filaments are of varying lengths so that said plurality of tissue grafts are spaced apart. ### Claim 47 (depends on 44) A process as defined in claim 44, wherein said elongate probe is arcuately curved. ### Claim 48 (depends on 44) A process as defined in claim 44, further comprising the step of closing said first and second openings after said filament is withdrawn from said mollusk. ### Claim 49 (depends on 44) A process as defined in claim 44, wherein soft tissue is the gonadal tissue of said mollusk. ### Claim 50 A process for nucleating pearls in shell-bearing molluska including abalone, comprising the steps of: (a) charging a hypodermic needle with a solution comprising nacre secretion stimulating material; (b) introducing said needle into a soft tissue of a host mollusk; (c) injecting said solution into said soft tissue to form a cavity therein, wherein said nacre secretion stimulating material is deposited on a portion of said soft tissue lining said cavity; (d) withdrawing said solution from said soft tissue to cause substantial collapse of said cavity; (e) charging said hypodermic needle with liquid epoxy resin; (f) injecting said liquid epoxy resin into said cavity; and (g) withdrawing said hypodermic needle from said soft tissue after a time period sufficient to allow said epoxy resin to set within said cavity to form a pearl nucleus therein. ### Claim 51 (depends on 50) A process as defined in claim 50, wherein said soft tissue is the adductor muscle of said mollusk and wherein said hypodermic needle is introduced into said muscle tissue through a hole formed in the shell of said mollusk in a region covering said muscle tissue. ### Claim 52 (depends on 51) A process as defined in claim 51, wherein said nacre secretion stimulating material comprises macerated molluska tissue. ### Claim 53 A process for nucleating pearls in shell bearing molluska including abalone, comprising the steps of: (a) forming an opening in the shell of a host mollusk in a region covering a soft tissue thereof; (b) providing a pearl nucleus of a size and shape sufficient to pass through said opening in a first orientation and capable of substantially occluding said opening in a second orientation; (c) manually inserting said pearl nucleus through said opening in said first orientation to a position adjacent said soft tissue; and (d) securing said nucleus in said second orientation against an inner surface of said shell adjacent said opening to substantially occlude said opening. ### Claim 54 (depends on 53) A process as defined in claim 53, further comprising the steps of: (a) fastening said pearl nucleus to a filament prior to insertion of said nucleus through said opening; (b) maintaining said nucleus in contact with said shell inner surface by pulling on said filament; and (c) severing said filament from said nucleus after said nucleus is secured to said shell. ### Claim 55 (depends on 53) A process as defined in claim 53, wherein said pearl nucleus comprises a disc portion adapted to engage said shell inner surface and a bead secured to said disc portion for deposition of nacre secreted by said molluska. ### Claim 56 A process for nucleating pearls in shell-bearing molluska including abalone, comprising the steps of: (a) forming a first opening in the shell of a host mollusk; (b) forming a second opening in the shell of said mollusk in a region covering a soft tissue thereof; (c) providing a pearl nucleus having a size and shape sufficient to pass through said first opening but not said second opening; (d) attaching one end of a filament to said pearl nucleus and the other end of said filament to an elongate probe; (e) inserting said probe through said first opening and passing said probe through an interior cavity of said mollusk between said soft tissue and said shell until said probe emerges through said second opening; (f) withdrawing said probe and attached filament through said second opening until said nucleus is lodged against the inner shell of said mollusk occluding said second opening; (g) securing said nucleus to said shell adjacent said second opening; and (h) severing said filament from said nucleus.