# US 5,347,075 A · Mutagenesis testing using transgenic non-human animals carrying test DNA sequences

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- Number: US 5,347,075 A
- Document ID: US5347075A
- Status: Granted
- Kind: A
- Application number: 07/711,332
- Filed: 1991-06-06
- Granted: 1994-09-13
- Earliest claimed: 1987-05-01
- Assignees: Stratagene
- Inventors: Joseph A. Sorge
- Web page: https://patentgrep.com/patent/US5347075A

## CPC

- C12N 15/8509: {for producing genetically modified animals, e.g. transgenic}
- A01K 67/0275: Genetically modified vertebrates, e.g. transgenic
- C12Q 1/02: involving viable microorganisms
- C12Q 1/18: Testing for antimicrobial activity of a material
- C12Q 1/6897: involving reporter genes operably linked to promoters
- G01N 33/5017: {for testing neoplastic activity}
- A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic)
- A01K 2227/105: Murine
- A01K 2267/0393: Animal model comprising a reporter system for screening tests
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

An assay for monitoring and assessing the mutagenic potential of agents which involves creating transgenic non-human animals carrying a test DNA sequence or sequences that can be quickly recovered and examined for mutations following exposure to one or more suspected mutagenic agents.

## Claims

### Claim 1

A transgenic mouse whose somatic and germ cells contain the beta-galactosidase gene flanked by the bacteriophage lambda cos sites, and the expression of said beta-galactosidase gene being capable of detection in E. coli.

### Claim 2 (depends on 1)

The transgenic mouse of claim 1, wherein said beta-galactosidase gene is carried in a modified bacteriophage lambda genome L2B.

### Claim 3 (depends on 2)

The transgenic mouse of claim 2 which is derived from a mouse embryo deposited with the American Type Culture Collection and having an ATCC accession number 72011.