# US 5,346,992 A · Process for isolating human albumin from supernatant IV, in particular IV-4, or from COHN's fraction V or from an analogous supernatant or fraction

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- Number: US 5,346,992 A
- Document ID: US5346992A
- Status: Granted
- Kind: A
- Application number: 07/832,081
- Filed: 1992-02-06
- Granted: 1994-09-13
- Earliest claimed: 1991-02-07
- Assignees: Pasteur Merieux Serums et Vaccins
- Inventors: Michel G. J. Grandgeorge; Jean-Luc B. Veron; Pierre L. J. Fournier
- Web page: https://patentgrep.com/patent/US5346992A

## CPC

- C07K 14/765: Serum albumin, e.g. HSA
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The process for isolating albumin from supernatant IV, and in particular IV-4, or from COHN's fraction V or from a plasma supernatant or fraction of analogous composition derived from an alcoholic or nonalcoholic fractionation, comprises one step on a hydrophilic anion exchange column with binding of albumin to the column, and then elution, and one step on a hydrophobic anion exchange column. Albumin thus obtained having a purity >99% by cellulose acetate electrophoresis, being free from impurities detected by crossed immunoelectrophoresis and free from polymers.

## Claims

### Claim 1

A process for isolating albumin having a purity greater than 99% with a polymer content lower than 1%, from a supernatant IV, or from COHN's fraction V derived from an alcoholic fractionation, comprising two anion exchange chromatographic steps, wherein one step is carried out on a hydrophilic anion exchange column with binding of albumin to the column followed by elution, and the other step is carried out on a hydrophobic anion exchange column.

### Claim 2 (depends on 1)

The process as claimed in claim 1, wherein the superinatant IV or the COHN's fraction V is passed through a chromatographic column containing porous silica beads coated with a hydrophilic polymer and then through a chromatographic column containing porous silica beads coated with a hydrophobic polymer.

### Claim 3 (depends on 7)

The process as claimed in claim 7, wherein the hydrophilic polymer is a dextran derivative and the hydrophobic polymer is a polyvinyltoluene derivative.

### Claim 4 (depends on 3)

The process as claimed in claim 3, wherein the hydrophilic polymer is a dextran bearing tertiary amine groups and the hydrophobic polymer is a polyvinyltoluene bearing quaternary ammonium groups.

### Claim 5 (depends on 2)

The process as claimed in claim 2, wherein the hydrophilic polymer is selected from the group consisting of agarose, hydrophilic polyvinyls and hydrophilic polyacrylamides.

### Claim 6 (depends on 2)

The process as claimed in claim 2, wherein the hydrophobic polymer is selected from the group consisting of agarose, polyvinyls and polyacrylamides made hydrophobic by grafting or co-crosslinking of hydrophobic groups.

### Claim 7 (depends on 1)

The process as claimed in claim 1, wherein the supernatant IV-4 or the COHN's fraction V is passed through columns containing a hydrophilic or hydrophobic organic polymer.

### Claim 8 (depends on 7)

The process as claimed in claim 7, wherein the polymer is selected from the group consisting of polysaccharides, polyvinyls, polyacrylamides and mixed polyacrylamide-agarose.

### Claim 9

A process for isolating albumin having a purity essentially of 100% with a polymer content essentially of 0%, from a supernatent IV, or from COHN's fraction V, comprising two anion exchange chromatographic steps, wherein one step is carried out on a hydrophilic anion exchange column with binding of albumin to the column, followed by elution, and the other is carried out on a hydrophobic anion exchange column.