# US 5,346,830 A · Gene expression system based on regulatory elements of bateriophage P2 and satellite phage P4

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- Number: US 5,346,830 A
- Document ID: US5346830A
- Status: Granted
- Kind: A
- Application number: 08/028,730
- Filed: 1993-03-08
- Granted: 1994-09-13
- Earliest claimed: 1991-06-11
- Assignees: Center for Innovative Technology
- Inventors: Gail Christie
- Web page: https://patentgrep.com/patent/US5346830A

## CPC

- C12N 15/70: Vectors or expression systems specially adapted for E. coli
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

An E. coli heterologous gene expression system comprising a multicopy expression vector comprising an F promoter from bacteriophage P2; and at least one copy of a DNA sequence comprising a delta gene from satellite P4 operably linked to a regulatable promoter on a different replicon is disclosed.

## Claims

### Claim 1

An E. coli heterologous gene expression system comprising:
a) a multicopy expression vector comprising an F promoter from bacteriophage P2; and
b) at least one copy of a DNA sequence comprising a delta gene from satellite bacteriophage P4 operably linked to a regulatable promoter.

### Claim 2 (depends on 1)

An E. coli heterologous gene expression system according to claim 1 further comprising a heterologous gene linked to said F promoter from bacteriophage P2.