# US 5,346,829 A · Device and method for detecting presence and extent of micro-organisms in a liquid sample or on a solid surface > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,346,829 A - Document ID: US5346829A - Status: Granted - Kind: A - Application number: 08/018,179 - Filed: 1993-02-16 - Granted: 1994-09-13 - Earliest claimed: 1992-02-16 - Inventors: Benjamin Alspector - Web page: https://patentgrep.com/patent/US5346829A ## CPC - C12M 23/04: {Flat or tray type, drawers (C12M23/10, C12M23/12, C12M23/16 take precedence)} - C12M 23/20: {Material Coatings (immunocoatings C12M25/00)} - C12M 23/22: {Transparent or translucent parts (glassware for laboratory use B01L3/00)} - C12M 33/00: {Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus (chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00)} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract A device is provided for detecting the presence of a microorganism in a liquid sample or on a solid surface that includes a cover member which is open at least at one end thereof; a support member accommodated within the cover member capable of carrying a microorganism culture medium on one or both opposite faces thereof, which support member is movable into and out from the cover member; a handle for moving the support member into and out from the cover member; and optionally having a sampling member. A combined method for detecting the presence and extent of microbial contamination in a liquid sample and inoculation of micro-organisms by employing the device is also disclosed. This involves transferring a portion of a liquid sample to the upper part of the culture medium and dipping the lower part of said culture medium in the liquid following the steps of dipping the tip in the liquid sample; removing the tips from the liquid; pushing the support member out of the cover member by the handle for moving the support member; dipping the front part of the exposed support member in the liquid sample so as to immerse the front part of the microbial culture medium carried on the support member in the liquid; removing the device from the liquid sample; pulling the support member back into the cover member by the handle for moving the support member; and incubating the device for microbial growth. ## Claims ### Claim 1 A device for detecting the presence of a microorganism in a liquid sample or on a solid surface, comprising: an elongated cover member which is open at least at one end thereof and having a slit in a wall of said cover member from said open end and extended along at least part of its longitudinal axis; an elongated support member accommodated within said cover member for carrying a microorganism culture medium on one or opposite surfaces thereof, which support member is movable into and out from said cover member; and means for facilitating movement of said support member into and out from said cover member comprising a handle attached to said support member at the one end thereof and projecting out of said cover member, which handle is movable within said slit, thereby sliding said support member into and out of said cover member through said open end. ### Claim 2 (depends on 1) A device according to claim 1 wherein said support member carries said culture member on opposite surfaces thereof. ### Claim 3 (depends on 1) A device according to claim 1 wherein said cover member has a rectangular cross section. ### Claim 4 (depends on 1) A device according to claim 1 wherein said cover member has an oval or polygonal cross section. ### Claim 5 (depends on 1) A device according to claim 1 wherein said support member is essentially flat, said culture medium being carried on the at least one flat surface thereof. ### Claim 6 (depends on 5) A device according to claim 5 where said support member has recesses on the at least one flat surface thereof, said culture medium being contained in said recesses. ### Claim 7 (depends on 1) A device according to claim 1 wherein said cove member is transparent. ### Claim 8 (depends on 1) A device according to claim 1 wherein said cover and support members are made from a plastic material. ### Claim 9 (depends on 1) A device according to claim 1 wherein said slit is narrower than the thickness of said support member. ### Claim 10 (depends on 1) A device according to claim 1 further comprising a non-integral cap, for covering the open end of said cover member. ### Claim 11 (depends on 1) A method for detecting the presence and extent of a microbial contamination in a liquid sample by employing the device according to claim 1 comprising the steps of: pushing said support member out of said cover member to expose said support member by pushing said handle forward along said slit; dipping said exposed support member in said liquid sample so as to immerse said culture medium carried on the support member in said liquid; removing the device from said liquid sample; pulling said support member back into said cover member by pulling said handle backward along said slit; and incubating the device for microbial growth. ### Claim 12 (depends on 1) A method for detecting the presence and extent of a microbial contamination on a solid surface by employing the device according to claim 1 comprising the steps of: pushing said support member completely out of said cover member by pushing said handle forward along said slit; contacting the exposed microbial culture on said support member with said solid surface; removing the device from the solid surface; pulling said support member back into said cover member by pulling said handle backward along said slit; and incubating the device for microbial growth. ### Claim 13 (depends on 1) A device according to claim 1 wherein said device is capable of quantitatively detecting the presence of a microorganism. ### Claim 14 A device for detecting the presence of a microorganism in a liquid sample or on a solid surface, comprising: an elongated cover member having an opening at least at one end thereof and having a slit in a wall of said cover member from said open end and extended along at least part of its longitudinal axis; an elongated support member accommodated within said cover member for carrying a microorganism culture medium on one or opposite surfaces thereof, which support member is movable into and out from said cover member through said at least one opening; means for facilitating movement of said support member into and out from said cover member comprising a handle attached to said support member at one end thereof and projecting out of said cover member, which handle is movable within said slit, thereby sliding said support member into and out of said cover member through said open end; and a sampling member attached to said cover member at said opening through which said support member is movable, comprising at lest one flexible tip extending outwardly from said cover member, which tip is bent inwardly, so that when the support member is pulled out from or pushed into said cover member said tip touches said culture medium, whereby a sample is smeared on said culture medium carried on said support member. ### Claim 15 (depends on 14) A device according to claim 14 wherein said sampling member comprises a frame to which said tips are attached, said frame having a cross section similar to that of the cover member and dimensioned so as to fit said cover member when attached thereto. ### Claim 16 (depends on 15) A device according to claim 15 wherein said sampling member comprise two or more flexible tips, on the same or opposite sides of said frame, which are bent inwards. ### Claim 17 (depends on 14) A device according to claim 14 wherein said sampling member is permanently or releasably hingedly attached to said cover member. ### Claim 18 (depends on 14) A method for detecting the presence and extent of a microbial contamination in a liquid sample and inoculation of microorganism by transferring a portion of said liquid sample onto a microbial culture medium, employing the device according to claim 14 comprising the steps of: dipping said tips in said liquid sample; removing said tips from the liquid sample; pushing said support member out of said cover member by pushing said handle forward along said slit for a distance sufficient to allow the tips to contact the support member; pulling said support member back into said cover member by pulling said handle backward along said slit; and incubating the device for microbial growth. ### Claim 19 (depends on 14) A method for detecting the presence and extent of a microbial contamination in a liquid sample and inoculation of microorganisms by transferring a portion of said liquid sample a part of said culture medium and dipping another part of said culture medium in said liquid employing the device according to claim 14 comprising the steps of: dipping said tips in said liquid sample; removing said tips from said liquid sample; pushing said support member out of said cover member by pushing said handle forward along said slit for a distance sufficient to allow the tips to contact the support member and that a part of the support member extend beyond the tips; dipping the extended part of said support member in said liquid sample so as to immerse said culture medium carried on the extended part of said support member in said liquid; removing the device from said liquid sample; pulling said support member back into said cover member pulling said handle backward along said slit; and incubating the device for microbial growth. ### Claim 20 (depends on 14) A method employing the device according to claim 14, comprising the steps of: dipping said tips in said liquid sample; removing the tips from said liquid sample; pushing said support member out of said cover member by pushing said handle forward along said slit to expose said support member; dipping a part of said exposed support member in said liquid sample so as to immerse said culture medium carried on the support member in said liquid; removing the device from the liquid sample; pulling said support member back into said cover member by pulling said handle backward along said slit; and incubating the device for microbial growth. ### Claim 21 (depends on 18) A method according to claim 18 wherein the sampling member is removed from the device after sampling. ### Claim 22 (depends on 14) A device according to claim 14 wherein said at least one flexible tip has a tapered end. ### Claim 23 (depends on 14) A device according to claim 14 wherein said sampling member is releasably attached to said cover member. ### Claim 24 (depends on 30) A device according to claim 30 wherein said elongated support member carries the culture medium on at least one surface thereof.