# US 5,346,821 A · Thermostable protease from Staphylothermus

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- Number: US 5,346,821 A
- Document ID: US5346821A
- Status: Granted
- Kind: A
- Application number: 07/949,491
- Filed: 1992-10-22
- Granted: 1994-09-13
- Earliest claimed: 1990-06-15
- Assignees: Novo Nordisk A/S
- Inventors: Garabed Antranikian; Michael Klingeberg
- Web page: https://patentgrep.com/patent/US5346821A

## CPC

- C12N 1/205: Bacterial isolates
- C11D 3/386: Preparations containing enzymes {, e.g. protease or amylase}
- C12N 9/52: derived from bacteria {or Archaea}
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

This invention is within the field of thermostable proteases. More specifically, the present invention relates to a thermosetable protease from Staphylothermus marinus, a process for the preparation of these enzymes, and detergent compositions comprising these enzymes. The enzyme has a temperature optimum in the range of from 90.degree.-100.degree. C. and a pH optimum in the range of from 6.5-10.

## Claims

### Claim 1

An isolated protease having the following properties:
(a) a pH optimum in the range of 6.5 to 10.0;
(b) a temperature optimum in the range of 90.degree. to 100.degree. C.; and
(c) immunochemical properties identical to those of the protease obtained from the strain Staphylothermus marinus, DSM No. 3639.

### Claim 2 (depends on 1)

The isolated protease according to claim 1 which is obtained from a strain of Staphylothermus.

### Claim 3 (depends on 2)

The isolated protease according to claim 2 which is obtained from a strain of Staphylothermus marinus.

### Claim 4 (depends on 3)

The isolated protease according to claim 3 which is obtained from the strain Staphylothermus marinus, DSM No. 3639.

### Claim 5 (depends on 1)

An isolated protease according to claim 1 which has a pH optimum in the range of 8.0 to 10.0

### Claim 6 (depends on 5)

An isolated protease according to claim 5 which has a pH optimum in the range of 8.5 to 9.5.

### Claim 7 (depends on 6)

An isolated protease according to claim 6 which has a pH optimum of around 9.0.

### Claim 8 (depends on 1)

An isolated protease according to claim 1 which has a temperature optimum of around 95.degree. C.

### Claim 9 (depends on 1)

A process for the preparation of the protease according to claim 1, comprising cultivating a protease producing strain of Staphylothermus in a suitable nutrient medium containing carbon and nitrogen sources and inorganic salts and recovering the protease.

### Claim 10 (depends on 9)

The process according to claim 9, wherein the protease producing strain is a strain of Staphylothermus marinus.

### Claim 11 (depends on 9)

The process according to claim 9, wherein the protease producing strain is Staphylothermus marinus, DSM 3639.

### Claim 12 (depends on 1)

A proteolytic detergent additive, comprising an isolated protease according to claim 1 in combination with a suitable excipient, said detergent additive being provided in the form selected from the group consisting of a non-dusting granulate, a liquid or a stabilized liquid, a slurry, and a protected enzyme.

### Claim 13 (depends on 1)

A detergent composition comprising an isolated protease according to claim 1 and a surfactant.

### Claim 14 (depends on 13)

The detergent composition according to claim 13, further comprising one or more other enzymes selected from the group consisting of an amylase, a lipase, a cellulase, and a peroxidase.