# US 5,346,819 A · Glycerol dehydrogenase, process for its production and its use

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 5,346,819 A
- Document ID: US5346819A
- Status: Granted
- Kind: A
- Application number: 08/019,676
- Filed: 1993-02-19
- Granted: 1994-09-13
- Earliest claimed: 1992-02-24
- Assignees: Toyo Boseki Kabushiki Kaisha
- Inventors: Osao Adachi; Kazunobu Matsushita
- Web page: https://patentgrep.com/patent/US5346819A

## CPC

- C12N 9/0006: {acting on CH-OH groups as donors (1.1)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Pyrroquinoline quinone-dependent glycerol dehydrogenase is provided which requires no surfactant for its solubilization and stabilization. The glycerol dehydrogenase has the following properties: (a) catalyzing the following reaction: glycerol+electron acceptor.dbd.glyceraldehyde+reduced electron acceptor, (b) optimum pH: pH 8-9, (c) pH stability: stable at pH 7-11, (d) optimum temperature: 20.degree.-25.degree. C., (e) thermal stability: stable at a temperature of 30.degree. C. or less for 10 minutes at a pH of 7.0, (f) molecular weight: 70,000 by gel filtration and SDS polyacrylamide gel electrophoresis, (g) bound to pyrroloquinoline quinone as a prosthetic group, (h) soluble and stable without the need of an ionic or non-ionic surfactant.

## Claims

### Claim 1

A purified glycerol dehydrogenase, which produces glyceraldehyde from glycerol, having the following properties:
(a) catalyzing the following reaction: glycerol+electron acceptor.dbd.glyceraldehyde+reduced electron acceptor,
(b) optimum pH: pH 8-9,
(c) pH stability: stable at pH 7-11,
(d) optimum temperature: 20.degree.-25.degree. C.,
(e) thermal stability: stable at a temperature of 30.degree. C. or less for 10 minutes at a pH of 7.0,
(f) molecular weight: 70,000 by gel filtration and SDS polyacrylamide gel electrophoresis,
(g) bound to pyrroloquinoline quinone as a prosthetic group,
(h) soluble and stable without the need of an ionic or nonionic surfactant.

### Claim 2 (depends on 1)

The purified glycerol dehydrogenase according to claim 1, wherein the electron acceptor is selected from the group consisting of potassium ferricyanide, 2,6-dichlorophenol-indophenol, Wurster's blue, and nitrotetrazolium blue.

### Claim 3 (depends on 1)

A process of producing the purified glycerol dehydrogenase of claim 1 which comprises cultivating, in a nutrient medium, a Pseudomonas sp. TE 3493 microorganism having the deposit number FERM BP-4169 and recovering said glycerol dehydrogenase from the culture without using an ionic or non-ionic surfactant.