Back to results
US 5,344,952 AGranted
Fluoroketone enzyme inhibitors
AssigneeBecton, Dickinson and Company
InventorsPatrick D. Mize, James P. O'Connell
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeBecton, Dickinson and Company
InventorsPatrick D. Mize, James P. O'Connell
Estimating the term…
A method for enzyme immunoassay for a ligand suspected to be present in a liquid sample includes signal amplification by use of at least two enzymes and a blocked modulator for one of the enzymes. Ligand present in the liquid binds to an antiligand and an enzyme-labeled tracer. The resulting bound fraction is separated and the enzyme in the tracer removes the blocking group from the blocked modulator. The modulator activates or inhibits a second enzyme which catalyzes the conversion of a substrate to a product. The presence or absence of the ligand in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the product. The invention includes a new class of enzyme inhibitors and blocked inhibitors and a kit of materials useful for performing the method of the invention.
A blocked enzyme inhibitor selected from the group of fluoroketones consisting of the general formulas I, II, III and IV ##STR7## wherein R.sub.1 is t-butyl or ##STR8## R.sub.2 is lower alkyl of 1-6 carbon atoms, Y and Z are independently H of F wherein at least one of Y and Z is F;
A blocked enzyme inhibitor of the formula ##STR10## where Y and Z are independently H or F wherein at least one of Y and Z are F;
A blocked enzyme inhibitor of the formula ##STR11## wherein n is 1-4;
Dependencies are inferred from the claim text.