# US 5,344,923 A · Nucleotide sequence encoding for bifunctional enzyme for proline production

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- Number: US 5,344,923 A
- Document ID: US5344923A
- Status: Granted
- Kind: A
- Application number: 07/953,695
- Filed: 1992-09-29
- Granted: 1994-09-06
- Earliest claimed: 1992-09-29
- Assignees: The Ohio State University Research Foundation
- Inventors: Desh P. S. Verma; Chien-an A. Hu; Ashton J. Delauney
- Web page: https://patentgrep.com/patent/US5344923A

## CPC

- C12N 9/0008: {acting on the aldehyde or oxo group of donors (1.2)}
- C12N 9/1217: {Phosphotransferases with a carboxyl group as acceptor (2.7.2)}
- C12N 15/8251: {Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis}
- C12N 15/8253: {Methionine or cysteine}
- C12N 15/8254: {Tryptophan or lysine}
- C12N 15/8273: {for drought, cold, salt resistance}
- C12P 13/24: Proline; Hydroxyproline; Histidine
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

The invention is directed to the isolation and identification of a mothbean (Vigna aconitifolia) cDNA clone encoding a bifunctional enzyme, delta.sup.1 -pyrroline-5-carboxylate synthetase, hereinafter P5CS, with both gamma-glutamyl kinase and glutamic-gamma-semialdehyde dehydrogenase activities that catalyzes the first two steps in plant proline production, also called biosynthesis.

## Claims

### Claim 1

An isolated DNA molecule having the sequence of SEQ ID NO.: 1.

### Claim 2

A cDNA clone encoding the enzyme of SEQ ID No. 1, a bifunctional enzyme that has both gamma-glutamyl kinase and glutamic-gamma-semialdehyde dehydrogenase activities.