# US 5,344,846 A · Compositions and methods for inhibiting deoxyhypusine synthase and the growth of cells

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- Number: US 5,344,846 A
- Document ID: US5344846A
- Status: Granted
- Kind: A
- Application number: 07/998,231
- Filed: 1992-12-30
- Granted: 1994-09-06
- Earliest claimed: 1992-12-30
- Assignees: The United States of America as represented by the Department of Health and Human Services
- Inventors: Judit Jakus; Myung H. Park; Edith C. Wolff; John E. Folk
- Web page: https://patentgrep.com/patent/US5344846A

## CPC

- A61K 31/155: Amidines ([IMAGE cpc-sch-A61K-1029.gif]), e.g. guanidine (H2N—C(=NH)—NH2), isourea (N=C(OH)—NH2), isothiourea (—N=C(SH)—NH2)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Compositions and methods for the treatment of mammalian cells to inhibit cell growth, especially for inhibiting the proliferative cell growth associated with malignant and non-malignant disease, are provided. More particularly, a deoxyhypusine synthase inhibitor, typically, a mono- or bisguanyl diamine or polyamine, is administered to the cells. Also provided by this invention are diagnostic methods and kits for screening the cells of a patient to determine the effect of the deoxyhypusine synthase inhibitor on proliferation of the cells.

## Claims

### Claim 1

A method for inhibiting the growth of mammalian cells, said method comprising administering a deoxyhypusine synthase inhibitor to the mammalian cells, wherein the deoxyhypusine synthase inhibitor is a compound having the general formula: ##STR1## wherein n is an integer from four to nine.

### Claim 2

A composition for inhibiting the growth of mammalian cells, which composition comprises a deoxyhypusine synthase inhibitor in a pharmaceutically acceptable carrier, wherein the deoxyhypusine synthase inhibitor is a compound having the general formula: ##STR2## wherein n is an integer from four to nine.

### Claim 3 (depends on 2)

A method for screening mammalian cells to determine their susceptibility to treatment with a deoxyhypusine synthase inhibitor comprising the steps of:
a) isolating the cells from a patient;
b) treating the cells with a deoxyhypusine synthase inhibitor composition of claim 2; and
c) determining the inhibitor's effect on the cells.

### Claim 4 (depends on 2)

A diagnostic kit comprising a deoxyhypusine synthesis inhibitor composition of claim 2 and other reagents, including a means for detecting the effectiveness of the inhibitor composition.

### Claim 5

A compound having the formula:
H.sub.2 N--(CH.sub.2).sub.n --NH--(C.dbd.NH)--NH.sub.2
where n is seven or eight.