# US 5,344,782 A · Assay method for the determination of lithium with novel substituted crown dyes

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- Number: US 5,344,782 A
- Document ID: US5344782A
- Status: Granted
- Kind: A
- Application number: 08/026,330
- Filed: 1993-03-04
- Granted: 1994-09-06
- Earliest claimed: 1986-05-23
- Assignees: Syntex (U.S.A.) Inc.
- Inventors: Cheng-I Lin; Marcel Pirio
- Web page: https://patentgrep.com/patent/US5344782A

## CPC

- C07D 273/00: Heterocyclic compounds containing rings having nitrogen and oxygen atoms as the only ring hetero atoms, not provided for by groups C07D261/00 - C07D271/00
- G01N 31/22: using chemical indicators (G01N31/02 takes precedence)
- G01N 33/84: involving inorganic compounds or pH
- Y10T 436/10: Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T 436/108331: Preservative, buffer, anticoagulant or diluent
- C07D: HETEROCYCLIC COMPOUNDS (macromolecular compounds C08)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

Assay methods are provided for determining the presence of lithium in high sodium content liquids such as serum, plasma, urine or other sample without deproteinization. The novel compounds are water soluble derivatives of TMC-crownformazans and provide signal enhancement by increased absorbance of the dye-lithium complex over the dye anion.

## Claims

### Claim 1

In a method for the determination of lithium in an aqueous body fluid sample having a concentration of sodium which interferes with the accuracy of said method, where said sample is not pretreated for deproteinization, comprising the steps of
(1) combining (a) a measured amount of an aqueous sample suspected of containing lithium and (b) a reagent comprised of a chromogenic ionophore and an aqueous buffer solution of pH of about 8 to about 14 to form an assay medium, wherein said chromogenic ionophore has the formula: ##STR5## wherein: R.sub.1 is an electron withdrawing group, selected from the group consisting of --NO.sub.3, --SO.sub.2 CF.sub.3, --CONX.sub.2, and --SO.sub.2 NX.sub.2, where X is independently selected from the group consisting of hydrogen, methyl and ethyl;
R.sub.2 and R.sub.5 are independently selected from the group consisting of hydrogen, methoxy, carboxymethoxy, nitro, chlorine, bromine and fluorine; and
R.sub.3 and R.sub.4 are independently selected from the group consisting of hydrogen and a hydrophilic substituent consisting of from one to twelve atoms other than hydrogen, which atoms are selected from the group consisting of carbon, oxygen, nitrogen, phosphorous, sulfur and halogen; with the proviso that not more than three of R.sub.3 and R.sub.4 are hydrogen; and
(2) measuring the absorbance of said medium, the improvement which comprises the inclusion of sodium ion in said assay medium containing said sample at a concentration at least equal to the sodium ion concentration introduced into said medium from said sample.