# US 5,344,777 A · Structural gene of membrane-bound alcohol dehydrogenase complex, plasmid containing the same and transformed acetic acid bacteria

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- Number: US 5,344,777 A
- Document ID: US5344777A
- Status: Granted
- Kind: A
- Application number: 07/985,458
- Filed: 1992-12-03
- Granted: 1994-09-06
- Earliest claimed: 1990-02-26
- Assignees: Nakano Vinegar Co., Ltd.
- Inventors: Toshimi Tamaki; Hiroshi Takemura; Kenji Tayama; Masahiro Fukaya; Hajime Okumura; Yoshiya Kawamura
- Web page: https://patentgrep.com/patent/US5344777A

## CPC

- C07K 16/40: against enzymes
- C12N 9/0006: {acting on CH-OH groups as donors (1.1)}
- Y10S 435/823: Acetobacter
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

There is provided a structural gene of membrane-bound alcohol dehydrogenase complex having a molecular size of about 7.0 kilo base which is derived from a microorganism belonging to the genus Acetobacter represented by Acetobacter altoacetigenes and shown by the nucleotide sequence of SEQ ID. NO. 1 and SEQ ID NO. 2. This enzyme increases the efficiency of acetic acid fermentation and may be effectively utilized for quantitative determination of alcohol.

## Claims

### Claim 1

A recombinant plasmid comprising
(a) vector pUC 18 having an ampicillin resistant gene derived from E. Coli,
(b) a cleaved DNA of pTA 5001 and
(c) a structural gene of membrane-bound alcohol dehydrogenase complex shown by FIG. 1 and having a molecular size of about 7.0 kilo bases comprising a protein having a molecular weight of about 72,000 as determined by SDS polyacrylamide gel electrophoresis, shown by nucleotide sequence SEQ ID NO. 1 and a protein having a molecular weight of about 44,000 as determined by SDS polyacrylamide gel electrophoresis, shown by nucleotide sequence SEQ ID NO. 2, which are derived from Acetobacter altoacetigenes.

### Claim 2 (depends on 1)

An acetic acid bacteria belonging to the genus Acetobacter transformed with the recombinant plasmid of claim 1.