# US 5,344,776 A · DNA encoding an insect octopamine receptor

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- Number: US 5,344,776 A
- Document ID: US5344776A
- Status: Granted
- Kind: A
- Application number: 07/676,174
- Filed: 1991-03-28
- Granted: 1994-09-06
- Earliest claimed: 1991-03-28
- Assignees: The United States of America as represented by the Department of Health and Human Services
- Inventors: John C. Venter; Claire M. Fraser; William R. McCombie
- Web page: https://patentgrep.com/patent/US5344776A

## CPC

- C07K 14/43581: {from Drosophila}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention pertains in general to invertebrate octopamine receptor proteins and to polynucleotides encoding such receptors. The present invention also relates to insect for example, Drosophila octopamine receptors that are recombinantly expressed in mammalian cells where the receptor mediates the attenuation of adenylate cyclase activity and exhibits a pharmacological profile that is unique but closely related to mammalian adrenergic receptors. The present invention further relates to drug screening methods for the development of specific human pharmacological drugs and insecticides targeted for the octopamine receptor system.

## Claims

### Claim 1

An isolated DNA segment selected from the group consisting of:
(a) a DNA segment encoding an invertebrate octopamine receptor protein comprising the amino acid sequence set forth in Sequence ID NO:2 and
(b) a DNA segment which is capable of hybridizing to the complement of a DNA segment according to (a) under low stringency hybridization conditions and which encodes an invertebrate octopamine receptor protein.

### Claim 2 (depends on 1)

The DNA segment according to claim 1 wherein said invertebrate is an insect.

### Claim 3 (depends on 1)

The DNA segment according to claim 1 wherein the invertebrate is Drosophila.

### Claim 4 (depends on 1)

The DNA segment according to claim 1 wherein said DNA segment has the nucleotide sequence shown in SEQ. ID. NO:1.

### Claim 5 (depends on 1)

A recombinant DNA molecule comprising
i) said DNA segment according to claim 1; and
ii) a vector.

### Claim 6 (depends on 5)

The recombinant DNA molecule according to claim 5 wherein said vector is a plasmid, bacteriophage or eucaryotic virus vector.

### Claim 7 (depends on 6)

The recombinant DNA molecule according to claim 6 wherein said vector is the plasmid expression vector pSVL.

### Claim 8 (depends on 7)

A mammalian cell containing the recombinant DNA molecule of claim 7.

### Claim 9 (depends on 8)

The mammalian cell according to claim 8 wherein said cell is CHO-K1 cell.

### Claim 10 (depends on 5)

A host cell containing the recombinant DNA molecule according to claim 5.

### Claim 11 (depends on 10)

The host cell according to claim 10 wherein said host cell is a mammalian cell.