# US 5,344,764 A · Protein inhibitors of phospholipase A.sub.2 purified from inflammatory sites and production process

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 5,344,764 A
- Document ID: US5344764A
- Status: Granted
- Kind: A
- Application number: 08/047,379
- Filed: 1993-04-16
- Granted: 1994-09-06
- Earliest claimed: 1989-08-03
- Assignees: Teijin Limited
- Inventors: Yorimasa Suwa; Atsushi Imaizumi; Masahiro Okada; Ichiro Kudo; Keizo Inoue; Yoji Suzuki
- Web page: https://patentgrep.com/patent/US5344764A

## CPC

- C07K 14/4703: {Inhibitors; Suppressors}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Protein inhibitors of phospholipase A.sub.2 purified from inflammatory sites which have an amino acid sequence given in FIGS. 3-1 through 3-3 or an amino acid sequence physiologically equivalent thereto, a process for preparation of said inhibitory protein wherein serum of mammalian animal is enzymatically treated, and a gene coding said inhibitory protein.

## Claims

### Claim 1

A process for preparing a phosphilipase A.sub.2 -inhibitory protein consisting of the amino acid sequence of FIGS. 3-1 through 3--3 comprising:
(a) obtaining human serum;
(b) incubating said serum at 37.degree. C. for 6 to 10 days;
(c) adding a protease inhibitor to said incubated serum; and
(d) purifying said inhibitory protein from said serum.

### Claim 2

Protein inhibitors of phospholipase A.sub.2 purified from inflammatory sites wherein said inhibitors consist of the amino acid sequence given in FIGS. 3-1 through 3-3.