# US 5,344,752 A · Plasma-based platelet concentrate preparations

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- Number: US 5,344,752 A
- Document ID: US5344752A
- Status: Granted
- Kind: A
- Application number: 08/043,574
- Filed: 1993-04-07
- Granted: 1994-09-06
- Earliest claimed: 1991-10-30
- Assignees: Thomas Jefferson University
- Inventors: Scott Murphy
- Web page: https://patentgrep.com/patent/US5344752A

## CPC

- A01N 1/125: Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
- A01N 1/10: Preservation of living parts
- A01N 1/126: Physiologically active agents, e.g. antioxidants or nutrients
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

The present invention provides a composition and method for improving the storage of platelets and optimizing the viability of stored platelets. The present invention allows platelets to be stored in plasma for extended periods, without the addition of buffer, by adding storage extension additives to a platelet concentrate.

## Claims

### Claim 1

A human blood, plasma-based platelet concentrate preparation for the storage of platelets consisting essentially of:
(a) platelets;
(b) plasma and anticoagulant, wherein said plasma and anticoagulant are present in an amount of at least 90 percent by weight of the concentrate preparation; and
(b) acetate, wherein said acetate is present in an amount of from about 5 to about 20 mM.

### Claim 2 (depends on 1)

The concentrate preparation of claim 1 wherein the acetate is present in an amount of from about 10 to about 20 mM.

### Claim 3 (depends on 1)

The concentrate preparation of claim 1 wherein the plasma and anticoagulant are present in an amount of at least about 95 weight percent of the concentrate preparation.

### Claim 4 (depends on 3)

The concentrate preparation of claim 3 wherein the acetate is present in an amount of from about 10 to about 20 mM.

### Claim 5 (depends on 4)

The concentrate preparation of claim 4 wherein the platelet concentration is from about 1.times.10.sup.6 to about 3.times.10.sup.6 platelets per microliter.

### Claim 6 (depends on 5)

The concentrate preparation of claim 5 wherein the plasma and anticoagulant are present in an amount of at least about 98 weight percent of the concentrate preparation.

### Claim 7

A human blood, plasma-based platelet concentrate preparation for the storage of platelets consisting of:
(a) platelets;
(b) plasma and anticoagulant, wherein said plasma and anticoagulant are present in an amount of at least 90 percent by weight of the concentrate preparation; and
(c) acetate, wherein said acetate is present in an amount of from about 5 to about 20 mM.

### Claim 8 (depends on 7)

The concentrate preparation of claim 7 wherein the acetate is present in an amount of from about 10 to about 20 mM.

### Claim 9 (depends on 7)

The concentrate preparation of claim 7 wherein the plasma and anticoagulant are present in an amount of at least about 95 weight percent of the concentrate preparation.

### Claim 10 (depends on 9)

The concentrate preparation of claim 9 wherein the acetate is present in an amount of from about 10 to about 20 mM.

### Claim 11 (depends on 10)

The concentrate preparation of claim 10 wherein the platelet concentration is from about 1.times.10.sup.6 to about 3.times.10.sup.6 platelets per microliter.

### Claim 12 (depends on 11)

The concentrate preparation of claim 11 wherein the plasma and anticoagulant are present in an amount of at least about 98 weight percent of the concentrate preparation.

### Claim 13

A human blood, plasma-based platelet concentrate preparation for the storage of platelets, produced by the process consisting essentially of the steps of suspending platelets in a solution consisting essentially of (i) at least about 90 weight percent plasma and anticoagulant for the plasma, and (ii) from about 5-20 mM acetate.

### Claim 14 (depends on 13)

The concentrate preparation of claim 13 wherein the acetate is present in an amount of from about 10-20 mM.

### Claim 15 (depends on 13)

The concentrate preparation of claim 13 wherein the plasma and anticoagulant are present in an amount of at least about 95 weight percent of the concentrate preparation.

### Claim 16 (depends on 15)

The concentrate preparation of claim 15 wherein the acetate is present in an amount of from about 10-20 mM.

### Claim 17 (depends on 16)

The concentrate preparation of claim 16 wherein the platelet concentration is from about 1.times.10.sup.6 to about 3.times.10.sup.6 platelets per microliter.

### Claim 18 (depends on 17)

The concentrate preparation of claim 17 wherein the plasma and anticoagulant are present in an amount of at least about 98 weight percent of the concentrate preparation.

### Claim 19

A human blood, plasma-based platelet concentrate preparation for the storage of platelets consisting of platelets, plasma and anticoagulant for the plasma, and acetate, wherein said acetate is present in an amount of from about 5 to about 20 mM.

### Claim 20 (depends on 19)

The concentrate preparation of claim 19 wherein the acetate is present in an amount of from about 17 to about 20 mM.