# US 5,344,647 A · Strain of microorganism having antimicrobial activity > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,344,647 A - Document ID: US5344647A - Status: Granted - Kind: A - Application number: 07/998,640 - Filed: 1992-12-29 - Granted: 1994-09-06 - Earliest claimed: 1987-01-21 - Assignees: Agricultural Genetics Company Limited - Inventors: Stephen Rossall - Web page: https://patentgrep.com/patent/US5344647A ## CPC - C12N 1/20: Bacteria; Culture media therefor - A01N 63/22: Bacillus - Y10S 435/839: Bacillus subtilis - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS ## Abstract The invention provides Bacillus subtilis strains NCIB 12375, NCIB 12376 and NCIB 12616, which have improved antimicrobial activity. The invention also relates to the use of such strains in the control of microbial infections and microbial contamination. ## Claims ### Claim 1 A biologically pure culture of Bacillus subtilis, Strain JB 3, a sample of which has been deposited at the National Collections of Industrial Bacteria Ltd. (NCIB) on 22 December 1986 under accession number NCIB 12375, or a mutant thereof having the same antimicrobial activity as the said strain. ### Claim 2 A biologically pure culture of Bacillus subtilis strain JB 3.6, a sample of which has been deposited at the National Collections of Industrial Bacteria Ltd. (NCIB) on 22 December 1986 under accession number NCIB 12376, or a mutant thereof having the same antimicrobial activity as the said strain. ### Claim 3 A biologically pure culture o Bacillus subtilis strain R1, a sample of which has been deposited at the National Collections of Industrial Bacteria Ltd. (NCIB) on 24 December 1987 under the accession number NCIB 12616 or a mutant thereof having the same antimicrobial activity as the said strain. ### Claim 4 An antimicrobial composition comprising a biologically pure culture of Bacillus subtilis strain JB 3, or JB 3.6, or R1, or a mutant of any of such strains, said mutant having the same antimicrobial activity as the said strain, or antimicrobial material isolated from any of such strains, together with a carrier or diluent therefor. ### Claim 5 (depends on 4) The antimicrobial composition according to claim 4, comprising a biologically pure culture of Bacillus subtilis strain JB 3, or a mutant thereof having the same antimicrobial activity as the said strain, or said antimicrobial material isolated from such strain. ### Claim 6 (depends on 4) The antimicrobial composition according to claim 4, comprising a biologically pure culture of Bacillus subtilis strain JB 3.6, or a mutant thereof having the same antimicrobial activity as the said strain, or said antimicrobial material isolated from such strain. ### Claim 7 (depends on 4) The antimicrobial composition according to claim 4, comprising a biologically pure culture of Bacillus subtilis strain R1, or a mutant thereof having the same antimicrobial activity as the said strain, or said antimicrobial material isolated from such strain. ### Claim 8 (depends on 4) A method of controlling microbial infections in plants, which comprises applying to the plants or their environment Bacillus subtilis strain JB 3, or JB 3.6, or R1, or a mutant of any of such strains, said mutant having the same antimicrobial activity as the said strain, or an antimicrobial composition according to claim 4. ### Claim 9 (depends on 4) The method of claim 4, comprising applying to said plants or their environment Bacillus subtilis strain JB 3, or a mutant thereof having the same antimicrobial activity as the said strain, or said antimicrobial material isolated from such strain. ### Claim 10 (depends on 8) The method of claim 8, comprising applying to said plants or their environment Bacillus subtilis strain JB 3.6, or a mutant thereof having the same antimicrobial activity as the said strain, or said antimicrobial material isolated from such strain. ### Claim 11 (depends on 8) The method of claim 8, comprising applying to said plants or their environment Bacillus subtilis strain R1, or a mutant thereof having the same antimicrobial activity as the said strain, or said antimicrobial material isolated from such strain. ### Claim 12 (depends on 1) The biologically pure culture of Bacillus subtilis according to claim 1, which is in a form suitable for use in control of microbial infection or microbial contamination. ### Claim 13 (depends on 2) The biologically pure culture of Bacillus subtilis according to claim 2, which is in a form suitable for use in control of microbial infection or microbial contamination. ### Claim 14 (depends on 3) The biologically pure culture of Bacillus subtilis according to claim 3, which is in a form suitable for use in control of microbial infection or microbial contamination. ### Claim 15 (depends on 4) The antimicrobial composition according to claim 4, also including a gum or surfactant. ### Claim 16 (depends on 15) The antimicrobial composition according to claim 15, which is in a form suitable for use in control of microbial infection or microbial contamination. ### Claim 17 (depends on 4) The antimicrobial composition according to claim 4, which is in a form suitable for use in control of microbial infection or microbial contamination. ### Claim 18 (depends on 8) The method of claim 8, wherein said antimicrobial composition further comprises a gum or surfactant. ### Claim 19 (depends on 9) The method of claim 9, wherein said antimicrobial composition further comprises a gum or surfactant. ### Claim 20 (depends on 10) The method of claim 10, wherein said antimicrobial composition further comprises a gum or surfactant. ### Claim 21 (depends on 11) The method of claim 11, wherein said antimicrobial composition further comprises a gum or surfactant.