# US 5,344,613 A · Agitation stabilizer in solid-phase peptide synthesizer

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- Number: US 5,344,613 A
- Document ID: US5344613A
- Status: Granted
- Kind: A
- Application number: 08/009,080
- Filed: 1993-01-26
- Granted: 1994-09-06
- Earliest claimed: 1992-01-29
- Assignees: Shimadzu Corporation
- Inventors: Kiyoshi Nokihara; Rintaro Yamamoto; Makoto Hazama; Shin Nakamura
- Web page: https://patentgrep.com/patent/US5344613A

## CPC

- B01L 99/00: Subject matter not provided for in other groups of this subclass
- B01J 19/0046: {Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00)}
- C07K 1/045: {using devices to improve synthesis, e.g. reactors, special vessels}
- B01J 2219/00286: Reactor vessels with top and bottom openings
- B01J 2219/00288: in the shape of syringes
- B01J 2219/005: Beads
- B01J 2219/0059: Sequential processes
- B01J 2219/00596: Solid-phase processes
- B01J 2219/00725: Peptides
- C40B 40/10: Libraries containing peptides or polypeptides, or derivatives thereof
- C40B 60/14: for creating libraries
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C40B: COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES (in silico combinatorial libraries of nucleic acids, proteins or peptides G16B35/00; in silico combinatorial chemistry G16C20/60)

## Abstract

In an automated solid-phase peptide synthesizing apparatus comprising at least one reaction vessel containing particulate resin as an insoluble support matrix which anchors elongating peptide chains yielded through coupling-assembly formation of peptide bonds, mixing agitation of reagents, as well as circulation agitation of washing solvents flushing the coupling-associated reaction solutions, introduced in peptide chain-assembly process steps into the reaction vessel, is effected by the bubbling of an inert gas forcibly passed through the reagents or washing solvent via a drainage port of the reaction vessel, in order to promote peptide synthesis. A removable agitation stabilizer is inserted into the reaction vessel as a barrier for inhibiting extra-vessel escape, due to action of the bubbling inert gas, of the reagents as well as the washing solvents, and of the support matrix anchoring the elongating peptide chains. The stabilizer also breaks clumping and coagulative skinning of elongated peptides bound to the support matrix, as effects further consequent of the action of the bubbling inert gas and levitated thereby toward the mouth of the reaction vessel during peptide synthesis. The stabilizer additionally acts as a baffle furthering mixing agitation, to promote dispersive mixing of the peptidyl resin with the reagents and washing solvents during peptide chain assembly; and It also serves to distribute washing solvents evenly along the inner surface of the reaction vessel.

## Claims

### Claim 1

A solid-phase peptide synthesizing apparatus, wherein peptide synthesis is promoted by means of bubbling agitation of reagents and washing solvents bathing an insoluble support matrix which anchors elongating peptide chains yielded through coupling-assembly formation of peptide bonds, comprising:
a reaction vessel having a drainage port, through which inert gas is forcibly introduced into a reaction chamber within said reaction vessel, in order to effect said bubbling agitation, said reaction vessel having further a supply opening, through which said reagents and said washing solvents are supplied into the reaction chamber, said reaction chamber further containing an insoluble support matrix sustained on a filter covering said port; and
a stabilizer removable from said reaction chamber positioned within said reaction chamber, for inhibiting splash of said reagents and washing solvents arising from said bubbling agitation, and further for breaking clumping and coagulative skinning of peptide-bound support matrix, due to flocculation effects abetted by said bubbling agitation during said coupling assembly of peptide chains, and levitated thereby in said reaction chamber;
said reaction vessel defining said reaction chamber being substantially cylindrical;
said stabilizer being partially formed as an annulus of diameter so corresponding to said reaction chamber as to allow said stabilizer to be concentrically accommodated within said reaction chamber;
said annulus of said stabilizer being supported by a leg in extension therefrom; and
said annulus also having a baffle leg in extension likewise as but shorter than said support leg, and bent radially inward relative to said annulus, for breaking clumping and coagulative skinning of said peptide-bound support matrix, due to said flocculation effects; wherein said baffle leg further promotes dispersive mixing, of said insoluble support matrix anchoring forming peptide chains with said reagents and washing solvents, during said peptide chain coupling assembly.

### Claim 2 (depends on 1)

A solid-phase peptide synthesizing apparatus according to claim 1, wherein said stabilizer is positioned so as to avoid interference with the supply of said reagents and washing solvents thereinto; said stabilizer furthermore being configured so as to avoid interference with said supply.

### Claim 3 (depends on 1)

A solid-phase peptide synthesizing apparatus according to claim 1, wherein said stabilizer support leg in installation penetrates said insoluble support matrix and is adjacent said filter within said reaction chamber.

### Claim 4 (depends on 3)

A solid-phase peptide synthesizing apparatus according to claim 3, wherein said supply of said reagents and washing solvents is through a tube a feed tip of which is contiguous with the reaction chamber near the supply opening of said reaction vessel, so as to obviate retention, due to capillarity at said feed tip, of said reagent or washing solvent after supply; wherein further
said annulus of said stabilizer effects circular distribution of said reagents and said washing solvents along said reaction chamber, thereby enhancing cleaning of said reaction chamber by said washing solvents.

### Claim 5 (depends on 1)

A solid-phase peptide synthesizing apparatus according to claim 1, wherein said stabilizer is configured as an O-ring, and is removably installed into the reaction chamber such that said O-ring, in a planar direction therethrough, is substantially parallel to the reaction chamber axially.

### Claim 6

In an automated solid-phase peptide synthesizing apparatus comprising at least one reaction vessel containing an insoluble support matrix for anchoring elongating peptide chains yielded through coupling-assembly formation of peptide bonds, and means for effecting mixing agitation of reagents and circulation agitation of washing solvents introduced in peptide chain coupling-assembly process steps into said reaction vessel through a mouth thereof, wherein an agitating material is forcibly passed through said reagents and washing solvents, via a drainage port provided in said reaction vessel, in order to promote both deprotecting reactions and peptide bond formation in said coupling assembly process, an improvement which comprises:
a stabilizer removable from said reaction chamber positioned within said reaction vessel such that said stabilizer acts as a barrier for inhibiting extra-vessel escape, due to action of said agitating material, of said reagents and said washing solvents, and of said insoluble support matrix anchoring said peptide chains elongating through said coupling assembly; and further for breaking clumping and coagulative skinning of peptide-bound support matrix, due to flocculation effects abetted by action of said agitating material therein and compelled thereby toward the mouth of said reaction vessel during said peptide chain coupling-assembly process; and wherein
said stabilizer is so configured as to avoid interference with the introduction into said reaction vessel of said reagent and said washing solvent through said mouth thereof, and further so as to promote dispersive mixing, of said insoluble support matrix anchoring said elongating peptide chains with said reagents and washing solvents, during said peptide chain coupling-assembly process;
said reaction vessel defining said reaction chamber being substantially cylindrical;
said stabilizer being partially formed as an annulus of diameter so corresponding to said reaction chamber as to allow said stabilizer to be concentrically accommodated within said reaction chamber;
said annulus of said stabilizer being supported by a leg in extension therefrom; and
said annulus also having a baffle leg in extension likewise as but shorter than said support leg, and bent radially inward relative to said annulus, for breaking clumping and coagulative skinning of said peptide-bound support matrix, due to said flocculation effects; wherein said baffle leg further promotes dispersive mixing, of said insoluble support matrix anchoring forming peptide chains with said reagents and washing solvents, during said peptide chain coupling assembly.