# US 5,344,571 A · Method for separating isoanalytes and measuring analytes in fluids

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- Number: US 5,344,571 A
- Document ID: US5344571A
- Status: Granted
- Kind: A
- Application number: 07/837,208
- Filed: 1992-02-18
- Granted: 1994-09-06
- Earliest claimed: 1992-02-18
- Assignees: University of Texas System Board of Regents
- Inventors: Philip B. Mendershausen; Vitauts Jurevics
- Web page: https://patentgrep.com/patent/US5344571A

## CPC

- G01N 1/4077: {by other techniques involving separation of suspended solids}
- B01D 61/18: Apparatus therefor
- G01N 35/08: using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
- G01N 1/34: Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)

## Abstract

An apparatus for separating isoanalytes and measuring analytes in fluids includes a two-chambered filtration device having a filter positioned parallel to the majority flow of fluids. The apparatus uses continuous flow filtration to separate one component from other fluid components. An alternative apparatus for separating isoanalytes and measuring analytes in fluids includes a reaction chamber which uses intermittent flow filtration with a piston/valve assembly to separate one component from other fluid components. A method for separating isoanalytes and measuring analytes in fluids comprises the steps of: mixing a fluid sample and a precipitating reagent; directing the mixture to a filtration device; adding a color reagent; directing the filtrate to an analyzer; replacing the fluid sample with a wash solution; and replacing the precipitating reagent with a cleaning solution. An alternative method for separating isoanalytes and measuring analytes in fluids comprises the steps of: directing a fluid sample to a reaction chamber; directing a precipitating reagent to the reaction chamber; mixing the fluid sample and the precipitating reagent; applying pressure to the reaction chamber; adding a color reagent to the filtrate; directing the filtrate to an analyzer; and replacing the fluid sample and the precipitating reagent with a cleaning solution.

## Claims

### Claim 1

Method for filtering components in a fluid sample, comprising the steps of:
directing a mixture comprising a sample and a precipitating reagent through a first chamber of a housing having first and second chambers and a filter therein;
substantially simultaneously directing a color reagent through the second chamber of the housing;
directing a sample across the filter into the second chamber;
mixing the filtered sample with the color reagent; and
subsequently directing a wash solution through the first chamber of the housing to remove residual sample therefrom.