Method and apparatus for measuring classes and subclasses of lipoproteins
AssigneeNorth Carolina State University
InventorJames D. Otvos
Life of this patent
Estimating the term…
Abstract
A method and apparatus for analyzing blood plasma or serum to determine the concentrations of its lipoprotein constituents includes obtaining the NMR chemical shift spectrum of a sample, Stored reference NMR spectra of the constituent subclasses of major lipoprotein classes are added together to form a lineshape that best fits the measured blood plasma NMR spectrum, and from this, the concentration of each lipoprotein constituent in the blood plasma or serum is determined.
Claims14
A method of measuring the lipoprotein constituents of blood, comprising:
- storing the NMR spectra of a plurality of lipoprotein classes as reference spectra for said classes, said storing step further comprising storing the NMR spectra of a plurality of subclasses for at least one of said lipoprotein classes as reference spectra for the subclasses of said lipoprotein class;
- acquiring an NMR signal produced by a plasma or serum sample in an NMR spectrometer;
- producing an NMR spectrum of the sample by transforming the acquired signal;
- aligning a control peak in the sample spectrum to a corresponding control peak in the reference spectra wherein said control peak responds to the environmental variables in the same manner as the lipoproteins;
- producing a calculated lineshape by adding together the stored reference spectra in amounts determined by respective non-negative reference spectrum coefficients;
- and
- adjusting the reference coefficients to fit the calculated lineshape to the NMR spectrum of the sample.
A method of measuring the lipoprotein constituents of blood, comprising:
- storing the NMR spectra of a plurality of lipoprotein classes as reference spectra for said classes, said storing step further comprising storing the NMR spectra of chylomicrons as reference spectra for chylomicrons;
- acquiring an NMR signal produced by a plasma or serum sample in an NMR spectrometer;
- producing an NMR spectrum of the sample by transforming the acquired signal;
- aligning a control peak in the sample spectrum to a corresponding control peak in the reference spectra wherein said control peak responds to the environmental variables in the same manner as the lipoproteins;
- producing a calculated lineshape by adding together the stored reference spectra in amounts determined by respective non-negative reference spectrum coefficients;
- and
- adjusting the reference coefficients to fit the calculated lineshape to the NMR spectrum of the sample.
An apparatus for measuring the lipoprotein constituents of blood, comprising:
- means for storing the NMR spectra of a plurality of lipoprotein classes as reference spectra for said classes, said storing step further comprising storing the NMR spectra of a plurality of subclasses for at least one of said lipoprotein classes as reference spectra for the subclasses of said lipoprotein class;
- means for acquiring an NMR signal produced by a plasma or serum sample in an NMR spectrometer;
- means for producing an NMR spectrum of the sample by transforming the acquired signal;
- means for aligning a control peak in the sample spectrum to a corresponding control peak in the reference spectra wherein said control peak responds to the environmental variables in the same manner as the lipoproteins;
- means for producing a calculated lineshape by adding together the stored reference spectra in amounts determined by respective non-negative reference spectrum coefficients;
- and
- means for adjusting the reference coefficients to fit the calculated lineshape to the NMR spectrum of the sample.
Dependencies are inferred from the claim text.