Panels of analyte-binding ligands
AssigneeTerrapin Technologies, Inc.
InventorLawrence M. Kauvar
Life of this patent
Estimating the term…
Abstract
The invention provides methods to obtain panels of low molecular weight polymers resulting from varying constituent monomers, which panels are systematically diverse with respect to two or more variables. The panels can be screened for members (paralogs) which are capable of specifically binding a target moiety. Such paralogs are useful for chromatographic separations and purifications of desired analytes and in binding assays, such as immunoassays, involving said analytes, as well as for any purpose which requires said specific binding. The invention also provides kits for these determinations, and methods to synthesize panels of candidate paralogs and use of these panels to generate analyte profiles and cross reactivity matrices.
Claims19
A panel for determining binding ability of a paralog to a target substance consisting essentially of individual candidate paralogs wherein said individual candidate paralogs have systematically varied values of at least two parameters over a maximal range to obtain maximal diversity in binding ability over the panel, each of which parameters determines the ability of the paralog to bind to other substances and/or wherein the combination of said parameters determines the ability of the paralog to bind to other substances.
A kit for the identification of a paralog useful for the binding to a target substance wherein
- said kit comprises a panel consisting essentially of individual candidate polymeric paralogs, wherein
- said individual candidate paralogs have systematically varied values of at least two parameters over a maximal range so as to obtain maximal diversity in binding ability over the panel, each of which parameters determines the ability of the paralog to bind to other substances, and/or wherein the combination of said parameters determines the ability of the paralog to bind to other substances.
A chromatographic support gradient containing paralogs which comprises a solid support having distributed thereon in a predetermined pattern at least a portion of the individual paralogs of a panel of individual paralogs wherein said individual paralogs have systematically varied values of at least two parameters over maximal range so as to obtain maximal diversity in binding ability over the panel, each of which parameters determines the ability of a paralog to bind to other substances and/or wherein the combination of said parameters determines the ability of the paralog to bind to other substances.
Dependencies are inferred from the claim text.