Process for producing a vaccine for a pathogenic RNA virus and product thereof
OwnerStephen E. Wrightinventor
Life of this patent
Estimating the term…
Abstract
A process for producing live, non-pathogenic, vaccines for the pathogens RNA turmor virus utilizes gene-altering technology to produce an altered genome which codes for the antigenic determinants of a pathogen, but has no genes coding for pathogenicity. The vaccine is the phenotypic expression of the altered genome. Specifically, an avian RNA tumor virus env gene is cloned into the non-pathogenic RNA virus RAV-O and the resulting recombinant product is replicated in host cells to provide a recombinant vaccine for the pathogen avian RNA tumor virus.
Claims4
A live, non-pathogenic vaccine for a pathogenic RNA virus, comprising an immunologically effective amount of a viral, antigenic, genomic construct having an antigenic determinant region of the RNA virus, but no pathogenic properties, the viral, antigenic, genomic construct being the RAV-Ac.sup.n virus.
A live, non-pathogenic, recombinant vaccine conferring immunity against the PrASV avian tumor virus in chickens, in which vaccine the PrASV env A gene has been inserted into a RAV-O virus by marker rescue to replace the endogenous envelope gene of the RAV-O virus, and the recombinant has been selected for in C/E cells.
A process for producing a live, non-pathogenic, recombinant vaccine conferring immunity against the PrASV avian tumor virus in chickens, comprising inserting the PrASV env A gene into a RAV-O virus by marker rescue such that said PrASV env A gene replaces the endogenous envelope gene of the RAV-O virus;
- and selecting for the recombinant in C/E cells.
Dependencies are inferred from the claim text.