CD4 adheson variants
AssigneeGenentech, Inc.
InventorsDaniel J. Capon, Timothy J. Gregory
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Abstract
Novel derivatives of cell surface proteins which are homologous to the immunoglobulin superfamily (adhesons) are provided. Amino acid sequence variations are introduced into adheson, the most noteworthy of which are those in which the transmembrane and, preferably, cytoplasmic domains are rendered functionally inactive, and in which adheson extracellular domains replace an immunoglobulin variable region. These variants are useful in therapy or diagnostics.
Claims12
A heterofunctional immunoadheson comprising a fusion protein in which a polypeptide comprising a human CD4 antigen variable (V) region is fused at its C-terminus to the N-terminus of a polypeptide comprising a constant region of an immunoglobulin chain disulfide bonded to a companion immunoglobulin heavy chain-light chain pair bearing a V.sub.L V.sub.H antibody combining site capable of binding a predetermined antigen.
A composition comprising a heterofunctional immunoadheson comprising a fusion protein in which a polypeptide comprising a human CD4 antigen variable (V) region is fused at its C-terminus to the N-terminus of a polypeptide comprising a constant region of an immunoglobulin chain disulfide bonded to a companion immunoglobulin heavy chain-light chain pair bearing a V.sub.L V.sub.H antibody combining site capable of binding a predetermined antigen.
A method of preparing a heterobifunctional immunoadheson comprising transfecting a host cell expressing nucleic acid encoding an immunoglobulin having a variable region directed against a predetermined antigen, with nucleic acid encoding a fusion protein in which a polypeptide comprising a human CD4 antigen variable (V) region is fused at its C-terminus to the N-terminus of a polypeptide comprising a constant region of an immunoglobulin chain, and culturing the resulting transfected host cell under conditions which permit the expression of said heterobifunctional immunoadheson by said transfected host cell.
Dependencies are inferred from the claim text.