Benzenetricarboxylate derivative-crosslinked low oxygen affinity hemoglobin
AssigneeDuke University
InventorsJoseph Bonaventura, Marius Brouwer, Robert E. Cashon
Life of this patent
Estimating the term…
Abstract
The invention is directed to a low oxygen affinity stroma-free tetrameric mammalian hemoglobin which is produced by crosslinkng with a derivative of benzenetricarboxylate, as well as methods for its preparation. Crosslinked stroma-free hemoglobin produced by methods of the present invention may be used in applications requiring physiological oxygen carriers such as in blood substitute solutions, or as in a plasma expander.
Claims9
Stroma-free tetrameric mammalian hemoglobin having an affinity for oxygen which is lower than that of non-crosslinked hemoglobin A, and which is crosslinked with a benzenetricarboxylate derivative, which derivative has at least two reactive sites capable of covalent bonding with and crosslinking of two peptide chains.
Stroma-free tetrameric mammalian hemoglobin having an affinity for oxygen which is lower than that of non-crosslinked hemoglobin A, and which is crosslinked with bis 1,3-(3,5-dibromosalicyl)benzenetricarboxylate.
A method for producing stroma-free crosslinked hemoglobin having an affinity for oxygen which is lower than that of non-crosslinked hemoglobin A comprising:
- (a) crosslinking hemoglobin with a benzenetricarboxylate derivative;
- and
- (b) isolating the crosslinked hemoglobin of step (a).
Stroma-free tetrameric mammalian hemoglobin having an affinity for oxygen which is lower than that of non-crosslinked hemoglobin A, and which is crosslinked with a crosslinking agent having the formula ##STR5## in which X.sub.1 and X.sub.2 are selected from the group consisting of H, Br, I, and NO.sub.2.
A method for producing stroma-free crosslinked hemoglobin having an affinity for oxygen which is lower than that of non-crosslinked hemoglobin A comprising:
- (a) crosslinking hemoglobin with a crosslinking agent having the formula ##STR6## in which X.sub.1 and X.sub.2 are selected from the group consisting of H, Br, I, and NO.sub.2 ;
- and
- (b) isolating the crosslinked hemoglobin of step (a).
A method for producing stroma-free crosslinked hemoglobin having an affinity for oxygen which is lower than that of non-crosslinked hemoglobin A comprising;
- (a) crosslinking hemoglobin with bis 1,3-(3,5-dibromosalicyl)benzenetricarboxylate;
- and
- (b) isolating the crosslinked hemoglobin of step (a).
Dependencies are inferred from the claim text.