Gold sol immunoassay system and device
AssigneeV-Tech, Inc.
InventorsJames E. Parker, Aileen Herranen
Life of this patent
Estimating the term…
Abstract
A new apparatus and method for immunoassays. A gold sol bead is held in a funnel member. First antibodies are associated with the gold sol bead. When the sample contacts the gold sol it dissolves the bead. A second antibody is impregnated on an immunosorbent surface. When the dissolved gold sol passes this surface, any antigen already reacted with the first antibody present reacts with the second antibody forming a gold: first antibody: antigen: second antibody: immunosorbent complex. The gold sol acts as the visible label.
Claims13
A system for one-step immunotesting of a liquid sample for the presence of a particular analyte, comprising:
- a funnel body having;
- a top liquid entrance and a bottom liquid exit;
- a cover having pipette access secured to the funnel body across the top liquid entrance;
- a filter secured to the funnel body across the bottom liquid exit at a bottom end of the funnel body;
- a gold sol bead within the funnel body, positioned between the cover and the filter, said gold sol bead comprising a particular first immunosorbent agent which is reactive with said particular analyte, to form a labelled immunosorbent-analyte complex,
- wherein said pipette access is an opening large enough for a pipette but smaller than the gold sol bead, and
- an immunosorbent assembly having;
- a body member having a means for removably receiving and supporting the bottom end of the funnel body;
- and
- an immunosorbent means secured within the body member below the means for receiving and supporting the bottom end of the funnel body, said immunosorbent means having, fixed thereto, a second immunosorbent agent complementary and reactive to said labelled first immunosorbent-analyte complex, such that when the labelled first immunosorbent-analyte complex comes into contact with said second immunosorbent agent, a labelled first immunosorbent-analyte-second immunosorbent complex is formed on said immunosorbent means.
A funnel body for one-step immunotesting comprising:
- a top liquid entrance and a bottom liquid exit;
- a cover having pipette access secured to the funnel body across the liquid entrance;
- a filter secured to the funnel body across the bottom liquid exit;
- and
- a gold sol bead, which includes binding partners that are specific for an analyte being tested for, within the funnel body, between the cover and the filter, wherein said pipette access is an opening large enough for a pipette but smaller than the gold sol bead.
An apparatus for one-step immunotesting of a liquid sample for the presence of a particular analyte comprising:
- a walled container having;
- a liquid entrance and a liquid exit for containing therewithin a labelled immunosorbent reagent bead comprising first reagent immunosorbent components having binding affinity for a particular immunoactive analyte, to form a labelled immunosorbent-analyte complex;
- a cover having pipette access secured to the walled container across the liquid entrance, wherein said pipette access is smaller than said reagent bead, and a filter secured to the walled container across the liquid exit at a bottom end of the walled container, said labelled immunosorbent reagent bead being positioned between the cover and the filter, and
- an immunosorbent assembly having;
- a body member a means for removably receiving and supporting the bottom end of the walled container;
- and
- an immunosorbent member secured within the body member below the means for receiving and supporting the bottom end of the walled container, said immunosorbent member having, fixed thereto, a second immunosorbent reagent complementary and reactive to said labelled immunosorbent-analyte complex, such that when the labelled immunosorbent-analyte complex comes into contact with said second immunosorbent reagent, a labelled immunosorbent-analyte-second immunosorbent complex is formed on said immunosorbent member.
Dependencies are inferred from the claim text.