Plasmid vector for expression in bacillus and used for cloning the structural gene which codes for the human growth hormone and a method of producing the hormone
AssigneeEniricerche S.p.A.
InventorsMarina Del Bue, Paola Cosmina, Elisabetta Franchi, Guido Grandi
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Abstract
The description relates to a plasmid vector expression in Bacillus and used for cloning the structural gene which codes for the human growth hormone, a recombinant DNA molecule comprising plasmid vector and the structural gene of the human growth hormone and a method of preparing the hormone comprising inserting the recombinant DNA molecule into strains of Bacillus and cultivating the resulting transformed strains in a suitable culture environment and recovering the thus-synthesized hormone from the cells.
Claims5
A microorganism transformed with the plasmid pSM212 which is stably maintained and which expresses the structural gene encoding human growth hormone at greater than or equal to 200 mg/l of human growth hormone, wherein the microorganism is of the genus Bacillus.
The microorganism B. subtilis pSMS108 (pSM212) ATCC 67097.
A plasmid vector which is stably maintained in Bacillus subtilis for high level expression of the structural gene encoding human growth at greater than or equal to 200 mg/l of human growth hormone hormone, wherein said gene is under the control of the promoter region having the following sequence:
- ##STR7##
Dependencies are inferred from the claim text.