Stably transformed coffee plant cells and plantlets
AssigneeEscagenetics Corporation
InventorsTommy L. Adams, Michael A. Zarowitz
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Abstract
Stably transformed Coffea arabica plant cells, derived from protoplasts and capable of regeneration, are disclosed.
Claims8
An isolated cell derived from a protoplast of the species Coffea arabica wherein the protoplast is derived from a cell line having all of the identifying characteristics of cell line CA-2 and is genetically transformed by means selected from the group consisting of electroporation and protoplast fusion to possess a stable trait wherein the cell can be maintained in cell culture and regenerated to a plantlet having the stable trait.
A cell derived from a protoplast of the species Coffea arabica wherein the protoplast is derived from a cell line having all of the identifying characteristics of cell line CA-2 and is genetically engineered to possess a stable trait and the cell can be maintained in cell culture and become a plantlet having the stable trait wherein the cell is obtained by:
- a) culturing Coffea arabica explant tissue to produce a callus,
- b) isolating a cell suspension comprising small cytoplasmically dense embryogenic cells from the callus,
- c) treating the cell suspension to obtain protoplasts,
- d) transforming the protoplasts, and
- e) regenerating cells from the transformed protoplasts.
Dependencies are inferred from the claim text.