Process for producing levan sucrase using Bacillus licheniformis
AssigneeSolvay Enzymes, Inc.
InventorsRobert L. Charles, Jayarama K. Shetty
Life of this patent
Estimating the term…
Abstract
The present invention relates to an acid stable levan sucrase enzyme which is not induced by sucrose. The present invention relates to a process for the preparation of this enzyme and microorganisms producing the levan sucrase enzyme. The invention also provides compositions containing this enzyme.
Claims11
A levan sucrase enzyme produced by Bacillus licheniformis APMC 84 having deposit number NRRL B-18962 and mutants thereof capable of producing said levan sucrase enzyme wherein said enzyme possesses at a temperature of about 55 C. a maximum activity at a pH of about 5.5 and retains at least 50% of its maximum activity at a pH of 4.0, as determined by the amount of glucose produced under the assay conditions which include sucrose as a substrate and measurement by high performance liquid chromatography.
A levan sucrase enzyme produced by Bacillus licheniformis APMC 84, having deposit number NRRL B-18962, and mutants thereof capable of producing said levan sucrase enzyme, wherein said enzyme possesses at a temperature of about 55 C. a maximum activity at a pH of about 5.5 and retains at least 50% of its maximum activity at a pH of 4.0, as determined by the amount of glucose produced under assay conditions which include sucrose as a substrate and measurement by high performance liquid chromatography, and wherein said levan sucrase enzyme is produced by a process comprising the steps of:
- (i) cultivating Bacillus licheniformis APMC 84 or mutants thereof in a suitable nutrient medium by forming a fermentation broth comprising a biomass;
- and
- (ii) recovering the levan sucrase enzyme from said fermentation broth,
- and wherein said Bacillus licheniformis APMC 84 or mutants thereof is cultivated in the absence of sucrose.
Dependencies are inferred from the claim text.