Process for purification of streptakinase using a reducing agent
AssigneeBeecham Group Limited
InventorsChristopher J. Mannix, Richard A. G. Smith, Ceri J. Lewis, Julian S. Harber
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Abstract
A process for the separation of streptokinase from contaminating proteins in a streptokinase-containing mixture, which comprises treating the mixture with a reducing agent to reduce disulphide bridges in the contaminating proteins to free thiol groups, contacting the mixture with a reagent R-X wherein R is a group capable of reacting with a free thiol group and X is a group R.sup.1 capable of reacting with a free thiol group or is a thiol-containing matrix, and thereafter separating the resulting chemically modified contaminating proteins from the mixture to provide streptokinase in a form substantially free of contaminating proteins.
Claims10
A process for the separation of streptokinase from contaminating proteins containing reducible disulphide bridges in a streptokinase-containing mixture, which comprises treating the mixture with a reducing agent to reduce said disulphide bridges in the contaminating proteins to free thiol groups, contacting the mixture with a reagent R-X wherein R is a group capable of reacting with a free thiol group and X is a group R.sup.1 capable of reacting with a free thiol group or is a thiol-containing matrix, and thereafter separating the resulting chemically modified contaminating proteins from the mixture to provide streptokinase in the form substantially free of said contaminating proteins.
Dependencies are inferred from the claim text.