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US 5,332,665 AGranted
Species specific, high affinity monoclonal antibodies
AssigneeThe General Hospital Corporation
InventorGuy L. Reed
Life of this patent
Estimating the term…
Abstract
High affinity species specific monoclonal antibodies are provided. The antibodies are anti-immunoglobulin antibodies which bind the Ig of a particular species but does not cross react with the Ig of another species. Methods for making and using the antibodies of the invention are provided.
Claims5
A method for screening a hybridoma supernatant for monoclonal antibodies which are completely species specific for the immunoglobulin of a first species (hereinafter "S1-Ig") comprising:
- (a) incubating said supernatant with S1-Ig and immunoglobulin from a second species (hereinafter "S2-Ig") wherein,
- (i) the concentration of S2-Ig is at least 100,000 times greater than the concentration of S1Ig;
- and
- (ii) the concentration of S2-Ig is equal to or greater than the concentration normally found in the serum of said second species;
- (b) after the incubation of step (a) , quantitating the amount of S1-Ig bound by antibody;
- and
- (c) selecting those supernatants having antibodies which bind S1-Ig despite the presence of S2-Ig.
4 more elements
Antibody 10B9 (ATCC deposit No. CRL 10946).
Dependencies are inferred from the claim text.