Back to results
US 5,332,664 AGranted
Human calcitonin precursor polyprotein structural gene
AssigneeCelltech Limited
InventorsRoger K. Craig, Iain MacIntyre
Life of this patent
Estimating the term…
Abstract
Production of human calcitonin precursor structural gene, the insertion of this gene in a vector system, the cloning thereof and a process for the production of authentic human calcitonin using recombinant DNA biotechnology.
Claims11
A process for the production of authentic human calcitonin comprising the steps of:
- i) preparing a polypeptide having the amino acid sequence of human calcitonin-X where -X is an amino acid residue attached by a peptide bond to the proline residue at the C-terminus of the human calcitonin 32 amino acid sequence and is enzymatically processable to --NH.sub.2 to produce C-terminal amidated authentic human calcitonin, by culturing a host organism transformed with a vector comprising a structural gene encoding said polypeptide;
- ii) recovering said polypeptide having the amino acid sequence of calcitonin-X;
- and
- iii) enzymatically processing -X to --NH.sub.2 in vitro, to produce authentic human calcitonin.
1 more element
A process for the production of authentic human calcitonin comprising the steps of:
- preparing a fusion polypeptide comprising a host polypeptide and a polypeptide having the amino acid sequence of human calcitonin-X, where -X is an amino acid residue attached by a peptide bond to the proline residue at the C-terminus of the human calcitonin 32 amino acid sequence and is enzymatically processable to --NH.sub.2 to produce C-terminal amidated authentic human calcitonin, by culturing a host organism transformed with a vector comprising a structural gene encoding said polypeptide;
- recovering said fusion polypeptide comprising a host polypeptide and a polypeptide having the amino acid sequence of human calcitonin-X;
- cleaving the fusion polypeptide;
- and enzymatically processing -X to --NH.sub.2 in vitro, to produce authentic human calcitonin.
1 more element
Dependencies are inferred from the claim text.