Process for the enzymatic preparation of 7-amino-cephalosporanic acids
AssigneeMinistero Dell'Universita' e Della Ricerca Scientifica e Tecnologica
InventorsEzio Battistel, Pietro Cesti, Giuliana Franzosi, Vilhelmus van der Goes
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Abstract
7-amino-cephalosporanic acids having the formula ##STR1## are obtained by submitting compounds having the following formula to enzymatic conversion: ##STR2## wherein R represents H, OH, O--CO--R", R" being an alkyl radical with from 1 to 4 carbon atoms, R.sup.1 represents a carboxylic group. The reaction is carried out in the presence of a microorganism selected from the Pseudomonas, Bacillus, Achromobacter genera, or in the presence of an enzyme, either free or immobilized, derived therefrom.
Claims7
A process for the enzymatic preparation of 7-amino-cephalosporanic acids having the formula ##STR5## wherein R represents H, OH, O--CO--R", R" being an alkyl radical with a number of carbon atoms ranging from 1 to 4, comprising selectively de-acylating compounds of the formula ##STR6## wherein R' is --COOH, by contacting said compound with a microorganism selected from the group consisting of:
- Achromobacter Xylosoxidans 13/D7, NCIMB 40407,
- Pseudomonas paucimobilis 4S/E7, NCIMB 40408,
- Pseudomonas sp. 13/F7, NCIMB 40409,
- Bacillus sp. 13/F8, NCIMB 40410,
- Bacillus cereus 146/1, NCIMB 40411
- Pseudomonas sp. 13/D7B, NCIMB 40473, and
- Pseudomonas sp. 146/H9, NCIMB 40474 or an acylase enzyme, in free form or immobilized, derived from said microorganism, and recovering said 7-amino-cephalosporanic acids.
Dependencies are inferred from the claim text.