Methods for determining peroxidatively active substances
AssigneeSyntex (U.S.A.) Inc.
InventorEdwin F. Ullman
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Abstract
Methods and compositions are disclosed for determining a peroxidatively active substance (PAS). The methods comprise the step of detecting a fluorescent signal produced upon cleavage of a compound of the formula F-L-Q, wherein F is a fluorester capable of producing the signal, Q is a quencher capable of quenching the signal when linked to F, and L is a bond, or a linking group having a bond, wherein the bond is capable of being cleaved by a reaction of the PAS with a substrate of the PAS and a hydrogen donor wherein the cleavage of the bond substantially reduces the quenching. The methods have application in a wide variety of systems including assays and improved assays for analytes. Also disclosed are kits for conducting the methods and improvements in accordance with the present invention.
Claims8
A kit for detecting a peroxidatively active substance (PAS) in a sample or medium suspected of containing said PAS, said kit comprising in packaged combination:
- a compound of the formula F-L-Q wherein F is a fluorescer capable of producing a fluorescent signal, Q is a quencher capable of quenching by energy transfer said signal when linked to F, and L is a bond or linking group containing a bond wherein said bond is capable of being cleaved by an oxidation-reduction reaction of said PAS with a substrate of said PAS and a hydrogen donor wherein said cleavage substantially reduces said quenching;
- and
- a hydrogen donor.
Dependencies are inferred from the claim text.