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US 5,332,660 AGranted
Gene encoding aspartic acid secretory proteinase of Candida albicans and a method of using the gene for the detection of Candida albicans
AssigneeTakara Shuzo Co., Ltd.
InventorsOsamu Takeda, Hiroyuki Mukai, Kiyozo Asada, Hideyo Yamaguchi, Ikunoshin Kato
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Abstract
This invention is directed to a gene sequence for secretory aspartic proteinase (SEQ ID No. 1) which is specific to pathogenic Candida yeast, to a highly sensitive method for detection of said gene in a sample solution and a kit used therefor.
Claims2
An isolated and purified gene from Candida albicans encoding a secretory aspartic acid proteinase and consisting of the DNA sequence described by SEQ ID No. 1.
A method for specific detection of Candida albicans in a biological sample comprising the following steps:
- (a) isolating a genomic DNA from said sample;
- (b) amplifying said genomic DNA with oligonucleotide primers using a polymerase chain reaction, wherein said primers are selected from the group consisting of the oligonucleotide pair having the sequence shown in the following Formulae 1-3:
- ##STR1## (c) detecting if specific amplification of said DNA has occurred as a means of detecting Candida albicans.
Dependencies are inferred from the claim text.