Capillary bed electrophoresis
AssigneeUniversity of Iowa Research Foundation
InventorsRavindra Datta, Robert A. Beardsley
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Abstract
A capillary bed electrophoresis device for separating and collecting large-scale electrophoretic samples is disclosed. The invention relates to an improvement in the anticonvective packing and cooling system comprising a series of capillaries through which the sample and an eluent are driven by force. The sample is separated via an electrical gradient established axially within the capillaries while a coolant may be circulated intimately around the exterior of said capillaries to remove any Joule heating. The separated components are then collected at the discharge end of the capillaries. The system may be used with either continuous or batch electrophoresis devices.
Claims20
A capillary bed device for large-scale separation and collection of components comprising:
- an elongated housing, defined by side walls having a first end disposed to receive a feed stream of a multi-component chemical sample and a second end disposed to discharge separated components;
- a plurality of spaced apart capillaries mounted within said housing and in fluid communication with the first end, and second end of the housing;
- a means for establishing an electrical gradient between the first and the second end of said housing and within the interior of said capillaries;
- a means for maintaining forced axial flow of an eluent through the interior of said capillaries, said eluent acting as a solvent and electrical conductor for said sample;
- and
- a means for feeding said sample into said housing and said capillaries.
A method of large-scale separation and collection of components by electrical charge, said method comprising:
- placing a multi-component sample in a feed receiving end of a capillary bed electrophoresis device;
- said electrophoresis device having an anticonvective packing comprising of plurality of axially disposed capillary tubes for receiving said sample;
- separating said components by their charge along an axial electrical gradient created within said tubes;
- forcing said components by bulk fluid flow of an eluent through said tubes by means of a pressure differential;
- and
- collecting said separated components at a sample discharge end of the capillary bed electrophoresis device.
A capillary bed device for large-scale separation and collection of components comprising:
- an elongated housing, defined by side walls having a first end disposed to receive a feed stream of a multi-component chemical sample and a second end disposed to discharge separated components;
- a plurality of spaced apart capillaries mounted within said housing and in fluid communication with the first end, and second end of the housing;
- an upper and a lower electrode positioned to maintain an electrical gradient between the ends of said housing and within the interior of said capillaries;
- a means for establishing said electrical gradient;
- a means for maintaining forced axial flow of an eluent through the interior of said capillaries, said eluent acting as a solvent and electrical conductor for said sample;
- and
- a means for feeding said sample into said housing and said capillaries.
A method of large-scale separation and collection of components by electrical charge, said method comprising;
- placing a multi-component sample in a feed receiving end of a continuous flow rotating annular capillary bed electrophoresis device;
- said electrophoresis device having an anti-convective packing comprising a plurality of axially disposed capillary tubes for receiving said sample;
- separating said components by their charge along an axial electrical gradient maintained within said tubes;
- forcing said components through said tubes by means of a pressure differential;
- circulating a coolant around the exterior of said capillaries;
- and
- collecting said separated components at an annularly discrete exit point at a sample discharge end of the capillary bed electrophoresis device.
Dependencies are inferred from the claim text.