Cross-linking collagenous product
AssigneeThe University of North Carolina at Chapel Hill
InventorGerald L. Mechanic
Life of this patent
Estimating the term…
Abstract
The present invention relates to a process for cross-linking a proteinaceous material. The process comprises: i) soaking the material to be cross-linked in an agueous solution of high osmolality; ii) incubating the material in an agueous buffer including an amount of a photooxidative catalyst sufficient to catalyze photooxidation of the material; and iii) irradiating the material and the catalyst of step (i) with light that includes a range of wavelengths selectively absorbed by the catalyst. Irradiation is effected under conditions such that cross-linking of the material occurs. In a further embodiment, the present invention relates to a cross-linked product produced by the above-described method.
Claims9
The stuble cross-linked collagenous product produced by the process comprising the steps of:
- soaking a collagenous tissue sample in an aqueous medium;
- immersing the soaked sample in an aqueous buffer including a photooxidative catalyst capable of donating electrons to the amino acids comprising the collagen fibrils of the collagenous tissue sample when excited by incident light to form either inter- or intramolecular or inter- and intra-molecular cross-links;
- and
- irradiating the soaked sample in an aqueous buffer including the catalyst with light while maintaining the oxygen concentration of the aqueous buffer so as to sensitize the catalyst into an excited state which is reduced by oxidative cross-linking of the amino acids comprising the collagenous tissue sample, the pH being maintained at between about 6.8 and about 8.6 and the temperature being maintained at between about -2.degree. and about 50.degree. C.
A stable cross-linked product produced by the method comprising the steps of:
- soaking collagen fibrils in a buffered medium having a neutral to alkaline pH which does not denature the collagen fibrils for a period of time sufficient to equilibrate the concentration of collagen with the concentration of a photooxidative catalyst solubilized in the medium, and
- thereafter oxidizing the collagen fibrils to form cross-linkages therebetween by inducing the transfer of electrons from the photooxidative catalyst to the collagen fibrils in the present of oxygen by exposing the collagen fibrils to light.
Dependencies are inferred from the claim text.