Methods and reagents for backcross breeding of plants
AssigneeLakeside Biotechnology, Inc.
InventorsLaurens J. Mets, Sharon A. Rogers, Mark J. Reichardt
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Abstract
This invention provides methods for more efficiently breeding novel species, subspecies or varieties of plants possessing desirable traits derived from other species, subspecies or varieties. In particular, the invention provides reagents and methods for quantitatively determining the genetic contribution from each parent in the progeny of a genetic cross between parental plants of different species, subspecies or varieties. The invention also provides methods for isolating hybrid and backcross progeny plants with a desired trait or combination of traits having inherited the minimum amount of DNA from the trait donor plant. Nucleic acid probes specific for species, subspecies and varieties of plants are also provided.
Claims11
A method for obtaining plants with a desired trait or combination of traits comprising:
- a. performing a genetic cross between a donor plant of a first species, subspecies or variety, having a desired trait or combination of traits and a recipient plant of a second species, subspecies or variety, respectively, to form a viable hybrid plant;
- b. backcrossing the hybrid plant of subpart (a) with a plant of the recipient species, subspecies or variety, respectively, to form a viable backcross progeny plant;
- c. quantitatively annealing DNA from individual backcross progeny plants produced in subpart (b) with a detectably-labeled probe or multiplicity of detectably-labeled probes that specifically binds to a plurality of probe binding sites in the DNA from the donor plant species, subspecies or variety and does not bind to the DNA of the recipient plant species, subspecies or variety, respectively;
- d. determining the amount of DNA in each backcross progeny plant inherited from the donor species, subspecies or variety, respectively, that binds to the detectably-labeled probe or multiplicity of detectably-labeled probes, scored as a pool and independent of a physical or genetic map of probe binding sites;
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- e. selecting for further propagation the backcross progeny plants having the desired trait or combination of traits that have inherited the least amount of DNA from the donor species, subspecies or variety.
Dependencies are inferred from the claim text.