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US 5,331,095 AGranted
Process for purification of basic fibroblast growth factor
AssigneeScios Nova Inc.
InventorsPaula J. Shadle, Kate B. Silverness, Robert S. King
Life of this patent
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Abstract
The invention relates to a method of purifying basic fibroblast growth factor. The method involves the use of strong cation exchange chromatography followed by hydrophobic interaction chromatography and then by weak cation exchange chromatography.
Claims9
A method to recover purified bFGF from a sample containing bFGF, said method consisting essentially of:
- (a) contacting a solution containing bFGF with a strong cation exchange matrix;
- (b) eluting from said strong cation exchange matrix a multiplicity of fractions at least one of which contains bFGF;
- (c) contacting the bFGF-containing strong cation exchange matrix fractions with a hydrophobic interaction matrix;.
- (d) eluting from said hydrophobic interaction matrix a multiplicity of fractions at least one of which contains bFGF;
- (e) contacting the bFGF-containing hydrophobic interaction matrix fractions with a weak cation exchange matrix;
- (f) eluting from said weak cation exchange matrix a multiplicity of fractions at least one of which contains bFGF;
- and
- (g) recovering the purified bFGF from the bFGF-containing weak cation exchange matrix fractions.
5 more elements
Dependencies are inferred from the claim text.