Process of preparation of collagen containing in major proportion insoluble collagen and collagen having high mechanical resistance and thermal stability obtained thereby
AssigneeColetica
InventorsAlain Huc, Rene Gimeno
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Abstract
A process of preparation of collagen containing in major proportion insoluble collagen is disclosed, including rinsing and washing the collagenic tissue, acidifying the ground material pH value 1 to 4 to get a collagenic paste which is diluted in a diluting solution to get the collagen gel having a concentration in collagen lower than about 2.5 by weight expressed in dry collagen, and performing a shearing stirring at high speed producing ultrasonic effect to get a substantially homogeneous collagenic gel. The collagen has improved mechanical resistance and thermal stability.
Claims17
A method of preparation of collagen from collagenic tissue, comprising grinding and optionally washing said collagenic tissue with a phosphate buffer and then with purified water to eliminate the phosphate, to get a ground material;
- acidifying said ground material to a pH value ranging between 3 and 4, so as to obtain a collagenic paste which is then diluted in a diluting solution to get a collagenic gel having a collagen concentration lower than 2.5% by weight expressed in dry collagen;
- and performing an homogenizing shearing stirring at high speed providing ultrasonic effect during a period of time sufficient to get a substantially homogeneous collagen gel containing in major proportion insoluble collagen.
Dependencies are inferred from the claim text.