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US 5,330,903 AGranted
Method for producing capsular polysaccharides
AssigneeCalifornia Institute of Technology
InventorsRoger G. Kern, Gene R. Petersen, Gil F. Richards
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Abstract
Structurally altered capsular polysaccharides are produced by mutant bacteria. These polysaccharides are isolated by selecting a wild type bacterial strain and a phage producing degradative enzymes that have substrate specificity for the capsular polysaccharides produced by the wild type bacteria. Phage-resistant mutants producing capsular polysaccharides are selected and the structurally altered capsular polysaccharide is isolated therefrom.
Claims9
A method for producing an isolated capsular polysaccharide comprising the steps of:
- (a) providing a wild type bacterial strain that produces a capsular polysaccharide and a bacteriophage that degrades at least one capsular polysaccharide produced by the wild type bacterial strain;
- (b) selecting phage resistant mutants of the bacterial strain that produce a capsular polysaccharide;
- (c) propagating the mutants to obtain a culture thereof;
- and;
- (d) harvesting the altering capsular polysaccharides of the mutants.
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