Method for the very rapid detection of polyamines
AssigneeDiagnostic Markers, Inc.
InventorsDavid Bar-Or, Clive Solomons
Life of this patent
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Abstract
A method for the detection of at least one polyamine associated with bacterial infection, which comprises contacting a sample suspected of containing polyamine-associated bacteria in aqueous medium at alkaline pH with indigo carmine and dimethyl sulfoxia and observing whether a blue color develops in five minutes indicating the presence of polyamine.
Claims16
A method for detecting the presence of a polyamine in a sample in which a blue color forms when the polyamine is present and a green color forms when the polyamine is absent, comprising the step of:
- contacting an aqueous sample containing a polyamine with a mixture of dimethyl sulfoxide and indigo carmine at an alkaline pH, wherein said blue color forms within five minutes indicating the presence of the polyamine.
A test kit for use in a method for the detection of a polyamine associated with bacterial vaginosis, which comprises the following separate components in a container:
- (1) a finely ground admixture of indigo carmine and sodium carbonate;
- (2) an aqueous solution of sodium chloride, potassium chloride, lithium chloride or urea;
- and
- (3) dimethyl sulfoxide, in amounts sufficient to produce a blue colored solution in the presence of a polyamine.
Dependencies are inferred from the claim text.