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US 5,329,000 AGranted
Purification of DNA with silicon tetrahydrazide
AssigneeBecton, Dickinson and Company
InventorsDaniel L. Woodard, Adriann J. Howard
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Abstract
A process for purifying DNA in which the DNA is bound to silicon tetrahydrazide in the presence of less than 2M chaotrope, low salt buffers, or water. The DNA is then eluted with low salt buffer or by heating in water.
Claims6
A process for purifying DNA which comprises:
- a) providing a silicon tetrahydrazide compound produced by reacting silicon tetrachloride with hydrazine to completion;
- b) binding the DNA to the silicon tetrahydrazide compound;
- and
- c) recovering the DNA from the silicon tetrahydrazide by eluting in TE buffer, TAE buffer, or TBE buffer or by heating the silicon tetrahydrazide compound with the bound DNA in water.
1 more element
Dependencies are inferred from the claim text.