US 5,328,999 AGranted

Endo-1,4-.beta.-glucanase genes and their use in plants

AssigneeThe Regents of the University of California

InventorsAlan B. Bennett, Robert L. Fischer, Coralie Lashbrook, James Giovannoni

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Abstract

The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense DNA constructions.

Claims7

Dependent claims

An expression cassette comprising a plant promoter sequence operably linked to a DNA subsequence of at least 20 base pairs derived from a DNA sequence encoding tomato a endo-1,4-.beta.-glucanase, the DNA subsequence being linked to the promoter sequence in the opposite orientation for expression.

Dependencies are inferred from the claim text.

Facts

Application: 07/687,466Filed 18 Apr 1991 · continuation in part
Priority: 20 Apr 1990
Granted: 12 Jul 1994
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 9/2437
Acting on beta-1,4-glucosidic bonds: cellulases
C12N 15/8249
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving ethylene biosynthesis, senescence or…
C12Y 302/01004
Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds: cellulase , i.e. endo-1,4-beta-glucanase
C12N
Microorganisms or enzymes

Family

Serial511,417Parent of this continuation-in-part · filed 20 Apr 1990
US 5,168,064 AParent of this continuation-in-part
US 5,328,999 AThis patent

From the US filing history. Foreign family members aren’t listed.

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