Preparation of alkali-modified cat dander allergen (Fel d I) for immunotherapeutic purposes
AssigneeImmuLogic Pharmaceutical Corporation
InventorMei-chang Kuo
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Abstract
Proteins, referred to as modified human T cell reactive feline proteins, which have reduced ability to bind immunoglobulin E from cat allergic individuals and substantially unaltered ability to stimulate T cells from cat allergic individuals (relative to affinity purified T cell reactive feline protein) and a method of making such proteins. The modified human T cell reactive feline proteins are useful in desensitization treatment of cat allergic individuals.
Claims11
A modified Fel d I, substantially free of other cat proteins, which is capable of stimulating T cells from a cat allergic individual and which interacts with human immunoglobulin E from a cat allergic individual to a lesser extent than does unmodified, affinity purified Fel d I, said modified Fel d I prepared by treating purified Fel d I with mild alkali.
A method for producing a modified Fel d I which is capable of stimulating T cells from a cat allergic individual and which interacts with human immunoglobulin E from a cat allergic individual to a lesser extent than does unmodified, affinity purified Fel d I, comprising the step of treating purified Fel d I with mild alkali.
A method for reducing the extent to which purified Fel d I interacts with human immunoglobulin E from a cat allergic individual without substantially reducing its capability to stimulate human T cells, comprising contacting purified Fel d I with mild alkali at a pH of about 12.0 to 14.0.
Dependencies are inferred from the claim text.