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US 5,328,990 AGranted
Isolation of macrophage migration inhibition factor from ocular lens
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorGraeme J. Wistow
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Abstract
Macrophage Migration Inhibition Factor (MIF) can be obtained from ocular lens of various birds and mammals. The amino acid sequences of lens MIF from mice, chickens and humans has been determined and the corresponding cDNA has been cloned.
Claims9
A method for obtaining Macrophage Migration Inhibition Factor (MIF) which comprises:
- homogenizing ocular lens in an aqueous solution to form a homogenate;
- separating a soluble extract containing MIF and an insoluble membrane fraction from said homogenate;
- and
- recovering purified MIF, having a molecular weight of 10-12 kDa, as determined by SDS polyacrylamide gel electrophoresis, from said soluble extract.
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Dependencies are inferred from the claim text.