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AssigneeSchering-Plough Corporation
InventorsGary Vellekamp, Susan Cannon-Carlson, John Tang
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A method for purifying Interleukin-10 (Il-10) from a eukaryotic cell culture medium. The method is comprised of subjecting IL-10 containing cell culture medium to cation exchange chromatography, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration chromatography. The present invention is also comprised of a process for separating different IL-10 dimers present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography. The present invention is also comprised of a process for separating variants of a protein differing in an N-terminal amino acid sequence present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography.
A method for purifying Interleukin-10 (IL-10) from a cell culture medium comprising:
A method for separating different dimers of IL-10 present in an IL-10 containing protein fraction comprising:
A method for separating different dimers of a protein contained within a protein fraction wherein the different dimers have different N-terminal amino acid sequences comprising:
A method for separating variants of a protein contained within a protein fraction wherein the variants of the protein have different N-terminal amino acid sequences comprising:
Dependencies are inferred from the claim text.