Method for allocating cells and cell allocation device

A cell allocation device for allocating cells on a culture substrate, comprising:

• first and second planar articles, each having front and back surfaces and a four-sided perimeter;
• and
• means for removably attaching the first and second planar articles to a culture substrate at respective angles to the culture substrate so that one side of each planar article perimeter is adjacent to the culture substrate and to each other to form a gap between the adjacent perimeter sides suitable for cells in a cell suspension to pass into the gap by capillary action for allocation on the culture substrate.

A cell allocation device for allocating cells on a culture substrate, comprising:

• first and second substantially L-shaped members, each L-shaped member including a pair of planar articles joined at respectively associated edges to form an L shape wherein each L-shaped member is arranged so that a planar article of each L-shaped member faces a corresponding planar article of the other L-shaped member;
• and
• spacer means for maintaining a gap suitable for admitting cells in a cell suspension by capillary action for allocation on the culture substrate between the facing planar articles of the first and second L-shaped members.

A cell allocation device for allocating cells on a culture substrate, comprising:

• an article having substantially planar first and second surfaces and at least one cell allocation zone including first zone walls and second zone walls, recessed from said first surface, wherein the walls of each zone slope downwardly from said first surface to respective apertures formed in the second surface;
• wherein said first and second sloped recessed zone walls and said apertures are arranged to conduct cells in a cell suspension down into the apertures by capillary action, to be allocated on the culture substrate.

A cell allocation device for allocating cells on a culture substrate, comprising:

• a floor, a perimeter wall contacting and extending at an angle to said floor, and a plurality of inner walls contacting and extending at an angle to said floor so as to define, with said floor, a plurality of chambers;
• wherein each of said chambers includes a cell allocation zone which is defined in part by a partition;
• and includes an aperture formed in the floor into which cells in a cell suspension pass by capillary action for allocation on the culture substrate, and through which said partition extends to contact the culture substrate.

A cell allocation device for allocating cells on a cell culture substrate, comprising:

• a plate material having a first slit through which cells are to be provided to a first zone on the cell culture substrate;
• wherein a slit is defined as an aperture having first and second linear coplanar dimensions, the first dimension being substantially longer than the second dimension such that the slit is suitable for cells in a cell suspension to pass thereinto by capillary action.

A cell allocation device, comprising:

• a plate material having a cutout connected throughout from a first surface of the plate material to a second surface of the plate material such that cells in a cell suspension pass into the cutout by capillary action, and a concavity provided in communication with said cutout for maintaining a cell suspension pool in the concavity.

A cell allocation device for allocating cells on a culture substrate, comprising:

• a plate material having first and second surfaces, a first concavity in the first surface of the plate material for maintaining a first cell suspension pool in the first concavity, a second concavity in the second surface of the plate material, and a first plurality of longitudinal cutouts connected in parallel between said first and second concavities, said first plurality of longitudinal cutouts having a construction such that cells in a cell suspension pass thereinto by capillary action when the second surface is in contact with the culture substrate.